| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Molecular Oncology, Markers, Clinical Correlates |
1 Institut für Humangenetik, Technische Universität München, München; 2 Institut für Humangenetik, GSF Forschungszentrum für Umwelt und Gesundheit, Neuherberg; and 3 Institut für Tumorbiologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany
Purpose: Metastases in distant organs are the major cause of death for cancer patients, and bone marrow is a prominent homing organ for early disseminated cancer cells. However, it remains still unclear which of these cells evolve into overt metastases. We therefore established a new approach based on the analysis of viable and proliferating cancer cells by single-cell comparative genomic hybridization.
Experimental Design: The bone marrow of early-stage breast tumor patients (pN0M0) was screened for tumor cells by immunostaining. By applying special short-term culturing, we selected for viable and proliferative tumor cells. The short-term culturing allowed us to evaluate the proliferative potential of micrometastatic cells, which we had previously shown to represent an independent prognostic marker. We assessed genomic changes in single disseminated cancer cells by single-cell comparative genomic hybridization.
Results: We found that these viable disseminated cancer cells already had a plethora of copy number changes in their genome. All of these cells showed chromosomal copy number changes with a substantial intercellular heterogeneity and differences to the matching primary tumors.
Conclusions: The established experimental strategy might pave the way for the identification of metastatic stem cells in cancer patients. Our preliminary results support the new concept that early disseminated cancer cells evolve independently from their primary tumor.
This article has been cited by other articles:
|
|
 |
|
  J. B. Geigl, A. C. Obenauf, J. Waldispuehl-Geigl, E. M. Hoffmann, M. Auer, M. Hormann, M. Fischer, Z. Trajanoski, M. A. Schenk, L. O. Baumbusch, et al. Identification of small gains and losses in single cells after whole genome amplification on tiling oligo arrays Nucleic Acids Res., June 18, 2009; (2009) gkp526v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Alix-Panabieres, S. Riethdorf, and K. Pantel Circulating Tumor Cells and Bone Marrow Micrometastasis Clin. Cancer Res., August 15, 2008; 14(16): 5013 - 5021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Vincent-Salomon, F C Bidard, and J Y Pierga Bone marrow micrometastasis in breast cancer: review of detection methods, prognostic impact and biological issues J. Clin. Pathol., May 1, 2008; 61(5): 570 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fiegler, J. B. Geigl, S. Langer, D. Rigler, K. Porter, K. Unger, N. P. Carter, and M. R. Speicher High resolution array-CGH analysis of single cells Nucleic Acids Res., February 16, 2007; 35(3): e15 - e15. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lacroix Significance, detection and markers of disseminated breast cancer cells Endocr. Relat. Cancer, December 1, 2006; 13(4): 1033 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Suzuki, E. S. Mose, V. Montel, and D. Tarin Dormant Cancer Cells Retrieved from Metastasis-Free Organs Regain Tumorigenic and Metastatic Potency Am. J. Pathol., August 1, 2006; 169(2): 673 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Pantel and U. Woelfle Circulating Tumor Cells as an Indicator of Invasion Am. Assoc. Cancer Res. Educ. Book, April 1, 2006; 2006(1): 116 - 119. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Braun, F. D. Vogl, B. Naume, W. Janni, M. P. Osborne, R. C. Coombes, G. Schlimok, I. J. Diel, B. Gerber, G. Gebauer, et al. A Pooled Analysis of Bone Marrow Micrometastasis in Breast Cancer N. Engl. J. Med., August 25, 2005; 353(8): 793 - 802. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
