This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Charpin, C. Articles by Bonnier, P. Search for Related Content PubMed PubMed Citation Articles by Charpin, C. Articles by Bonnier, P.

Tumor Neoangiogenesis by CD31 and CD105 Expression Evaluation in Breast Carcinoma Tissue Microarrays

¹ Department of Pathology, Centre Hospitalier et Universitaire Nord, and ² Department of Gynecologic Oncology, Hôpital de La Conception, Marseille, France

Purpose: The aim of the study was to evaluate CD31 and CD105 immunohistochemical expressions in tissue microarrays from 360 breast carcinomas.

Study design: Computerized (ACIS/Chromavision) assisted image analysis was performed to compare immunoreactions in tissue microarrays with those in current paraffin and frozen sections. We also aimed to determine the CD105 and CD31 prognostic significance and relevance in routine practice by correlating results of immunodetections with patients’ (n = 360) outcome (14.3-year follow-up).

Results: The results show (a) that in tissue microarrays, the CD31 and CD105 expression quantified by image analysis device did not correlate with the measurements assessed on routine paraffin sections; (b) that CD105 expression is endowed of a prognostic significance in paraffin sections in terms of overall survival (P < 0.01), whereas in contrast, CD31 on paraffin sections did not correlate with patients overall survival; (c) that semiquantitative analysis of CD105 expression correlated with the image analysis measurements in frozen sections ( = 0.671, P < 0.01) and paraffin ( = 0.824, P < 0.01) sections. However, paraffin sections were less immunostained than frozen ones.

Conclusions: It is concluded (a) that CD105 may be suitable in paraffin sections to evaluated neoangiogenesis; and (b) that tissue microarrays are not suitable substrates for neoangiogenesis evaluation as a prognostic indicator in breast carcinomas, in contrast to current tissue sections.

This article has been cited by other articles:

				H. P. Dhakal, B. Naume, M. Synnestvedt, E. Borgen, R. Kaaresen, E. Schlichting, G. Wiedswang, A. Bassarova, K.-E. Giercksky, and J. M. Nesland Vascularization in Primary Breast Carcinomas: Its Prognostic Significance and Relationship with Tumor Cell Dissemination Clin. Cancer Res., April 15, 2008; 14(8): 2341 - 2350. [Abstract] [Full Text] [PDF]

				Y. Yao, Y. Pan, J. Chen, X. Sun, Y. Qiu, and Y. Ding Endoglin (CD105) Expression in Angiogenesis of Primary Hepatocellular Carcinomas: Analysis using Tissue Microarrays and Comparisons with CD34 and VEGF Ann. Clin. Lab. Sci., January 1, 2007; 37(1): 39 - 48. [Abstract] [Full Text] [PDF]

				X. Yao, C.-N. Qian, Z.-F. Zhang, M.-H. Tan, E. J. Kort, X. J. Yang, J. H. Resau, and B. T. Teh Two Distinct Types of Blood Vessels in Clear Cell Renal Cell Carcinoma Have Contrasting Prognostic Implications Clin. Cancer Res., January 1, 2007; 13(1): 161 - 169. [Abstract] [Full Text] [PDF]