| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Molecular Oncology, Markers, Clinical Correlates |
1 Division of Endocrinology, Diabetes, and Metabolism, Departments of Medicine, Biological Chemistry, and Pharmacology, University of California, Irvine, California; 2 Bruce Rappaport Faculty of Medicine, Technion, Israel Institute of Technology, Haifa, Israel; and 3 The First Department of Surgery, Yamanashi Medical University, Yamanashi, Japan
Purpose: Neuropilin (Np)-1 and -2 are coreceptors for vascular endothelial growth factor (VEGF). This study was designed to assess their role in pancreatic ductal adenocarcinoma (PDAC).
Experimental Design: We assessed Np-1 and Np-2 expression by real-time quantitative PCR in relation to the expression of VEGF ligands and receptors in pancreatic cancer cell lines and tissues.
Results: ASPC-1, CAPAN-1, and PANC-1 pancreatic cancer cells and tumor-derived, laser-captured pancreatic cancer cells exhibited higher Np-1 and Np-2 mRNA levels than VEGF receptor-1, -2, or -3 mRNA levels. Transfection of Np-1 and Np-2 cDNAs in COS-7 cells, and treatment with tunicamycin revealed that both proteins were glycosylated. Both proteins were expressed in pancreatic cancer cell lines, in the PDAC samples, and in acinar cells adjacent to the cancer cells. The normal pancreas was devoid of Np-1 immunoreactivity, whereas Np-2 immunoreactivity was present in the endocrine islets and in some acinar cells, but not in ductal cells.
Conclusions: The aberrant localization of Np-1 and Np-2 in the cancer cells in PDAC suggests that in addition to exerting proangiogenic effects, these coreceptors may contribute to novel autocrine-paracrine interactions in this malignancy.
This article has been cited by other articles:
|
|
 |
|
  N. A. Dallas, M. J. Gray, L. Xia, F. Fan, G. van Buren II, P. Gaur, S. Samuel, S. J. Lim, T. Arumugam, V. Ramachandran, et al. Neuropilin-2-Mediated Tumor Growth and Angiogenesis in Pancreatic Adenocarcinoma Clin. Cancer Res., December 15, 2008; 14(24): 8052 - 8060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Matsushita, T. Gotze, and M. Korc Hepatocyte Growth Factor Mediated Cell Invasion in Pancreatic Cancer Cells Is Dependent on Neuropilin-1 Cancer Res., November 1, 2007; 67(21): 10309 - 10316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Gaspar, L. Li, A. M. Kapoun, S. Medicherla, M. Reddy, G. Li, G. O'Young, D. Quon, M. Henson, D. L. Damm, et al. Inhibition of Transforming Growth Factor beta Signaling Reduces Pancreatic Adenocarcinoma Growth and Invasiveness Mol. Pharmacol., July 1, 2007; 72(1): 152 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Ellis The role of neuropilins in cancer Mol. Cancer Ther., May 1, 2006; 5(5): 1099 - 1107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. von Wronski, N. Raju, R. Pillai, N. J. Bogdan, E. R. Marinelli, P. Nanjappan, K. Ramalingam, T. Arunachalam, S. Eaton, K. E. Linder, et al. Tuftsin Binds Neuropilin-1 through a Sequence Similar to That Encoded by Exon 8 of Vascular Endothelial Growth Factor J. Biol. Chem., March 3, 2006; 281(9): 5702 - 5710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. El-Sheikh, P. Borgstrom, G. Bhattacharjee, M. Belting, and T. S. Edgington A Selective Tumor Microvasculature Thrombogen that Targets a Novel Receptor Complex in the Tumor Angiogenic Microenvironment Cancer Res., December 1, 2005; 65(23): 11109 - 11117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Bertelli and M. Bendayan Association between Endocrine Pancreas and Ductal System. More than an Epiphenomenon of Endocrine Differentiation and Development? J. Histochem. Cytochem., September 1, 2005; 53(9): 1071 - 1086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Korc Targeted Therapeutics in Pancreatic Cancer: A Ray of Hope Clin. Cancer Res., January 1, 2005; 11(1): 410 - 411. [Full Text] [PDF]
|
|
|
|
|
|
M. Fukasawa and M. Korc Vascular Endothelial Growth Factor-Trap Suppresses Tumorigenicity of Multiple Pancreatic Cancer Cell Lines Clin. Cancer Res., May 15, 2004; 10(10): 3327 - 3332. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
