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MIB-1 (KI-67) Proliferation Index and Cyclin-Dependent Kinase Inhibitor p27^Kip1 Protein Expression in Nephroblastoma

¹ Departments of Pediatric Urology and ² Pathology, Josephine Nefkens Institute; ³ The Netherlands Institute for Health Sciences; ⁴ Department of Pediatric Oncology, Erasmus MC-University Medical Center Rotterdam, Rotterdam, the Netherlands; and ⁵ Urology Department, Menoufiya University, Menoufiya, Egypt

Purpose: A number of studies have indicated that the tumor proliferation marker MIB-1 and cell cycle inhibitor p27^Kip1 expression are of prognostic importance in a variety of cancers. The present study was performed to evaluate the prognostic value of these molecules in Wilms’ tumors.

Experimental Design: MIB-1 and p27^Kip1 expressions were investigated by the means of immunohistochemical analysis of 62 Wilms’ tumor. Patients were preoperatively treated by chemotherapeutic agents and had a mean follow-up of 5.7 years.

Results: MIB-1 and p27^Kip1 were expressed in normal kidney tissues and in the three main components of Wilms’ tumor, i.e., the blastemal, epithelial, and stromal cells. In Wilms’ tumors, the percentage of MIB-1-positive cells in the blastema ranged between 0 and 42% (mean, 9.4%) and in the epithelial component between 0 and 53% (mean, 19.9%), with a significant difference (P < 0.01). The percentage of blastemal p27^Kip1-positive cells ranged between 3 and 85% (mean, 55.1%) and for the epithelial component between 1 and 87% (mean, 59%). There was a significant inverse relationship between blastemal MIB-1 and p27^Kip1 expression in Wilms’ tumor. Univariate analysis showed that blastemal MIB-1 and p27^Kip1 expression were indicative for clinical progression and tumor-specific survival. In a multivariate analysis, blastemal MIB-1 and p27^Kip1 protein expression proved to be an independent prognostic for clinical progression besides stage.

Conclusions: It was concluded that both MIB-1-based proliferative activity and p27^Kip1 protein expression in the blastema have prognostic impact in Wilms’ tumor.

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				C. Jones, K. Pritchard-Jones, M. A. Ghanem, T. H. Van der Kwast, R. M. Nijman, and G. J. van Steenbrugge MIB-1 and p27Kip1 Expression in Nephroblastoma Clin. Cancer Res., November 15, 2004; 10(22): 7785 - 7786. [Full Text] [PDF]