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Molecular Oncology, Markers, Clinical Correlates |
Departments of 1 Pathology and 2 Surgery, Queen Elizabeth Hospital, Hong Kong Special Administrative Region, and Departments of 3 Chemical Pathology and 4 Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong Special Administrative Region, Hong Kong, China
ABSTRACT
Purpose: Colorectal cancer is an important cause of cancer deaths. Here, we focused our investigation on the ß-catenin gene which is implicated in colorectal carcinogenesis and tested whether ß-catenin mRNA is detectable in the plasma of colorectal carcinoma and adenoma patients using quantitative reverse transcriptase-PCR.
Experimental Design: Plasma ß-catenin mRNA was measured from 58 colorectal carcinoma patients, 49 colorectal adenoma patients, and 43 apparently normal subjects using intron-spanning primers and Taqman probes. Five clinicopathological parameters were studied and correlated with plasma ß-catenin mRNA concentration. Additionally, 19 colorectal carcinoma patients after tumor removal were also recruited for plasma ß-catenin mRNA measurement to further demonstrate the clinical usefulness of this test.
Results: ß-catenin mRNA was detected with median concentrations of 8737 (range: 1480–933,100), 1218 (range: 541–2,254) and 291 (range: 0–1,366) copies/ml plasma in colorectal carcinoma, colorectal adenoma, and apparently normal subjects, respectively. Statistical analysis demonstrated that plasma ß-catenin mRNA concentration was correlated to tumor stage but not sex, age, lymph node status, and degree in differentiation. Moreover, plasma ß-catenin mRNA concentration decreased significantly after tumor removal in 16 of 19 (84%) colorectal carcinoma patients.
Conclusions: We conclude that plasma ß-catenin mRNA may potentially serve as a marker for colorectal cancer.
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