This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yamanaka, N. Articles by Katano, M. Search for Related Content PubMed PubMed Citation Articles by Yamanaka, N. Articles by Katano, M.

Interleukin 1ß Enhances Invasive Ability of Gastric Carcinoma through Nuclear Factor-B Activation

Departments of ¹ Cancer Therapy and Research, ² Anatomic Pathology, and ³ Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan

Purpose: We examined the role of interleukin (IL)-1ß in activation of nuclear factor B (NF-B) and the biological function of activated NF-B in gastric carcinoma cells.

Experimental Design: Human gastric carcinoma cell line GCTM-1 was used to examine NF-B activation by immunostaining and electrophoretic mobility shift assay. Matrix metalloproteinase (MMP)-9 expression, which plays an important role in tumor invasion, was assessed by semiquantitative reverse transcription-PCR, Western blotting, and immunostaining. The invasive ability of GCTM-1 cells was measured by Matrigel invasion assay. In vivo expression of IL-1ß and MMP-9 and activation of NF-B in 10 surgically resected gastric carcinoma specimens were examined immunohistochemically.

Results: IL-1ß enhanced NF-B activation, MMP-9 expression, and the invasive ability of GCTM-1. A NF-B inhibitor, pyrrolidine dithiocarbamate, suppressed both MMP-9 expression and invasiveness of IL-1ß-treated GCTM-1 cells. IL-1ß did not increase the invasive ability of GCTM-1 cells transfected with MMP-9 antisense oligonucleotide. Concomitant expression of IL-1ß and nuclear NF-B was observed in 3 of 10 gastric carcinoma specimens. Cells producing IL-1ß were tumor-infiltrating macrophages in two specimens and gastric carcinoma cells in one specimen.

Conclusions: One of the molecules that may play a role in NF-B activation in some gastric carcinomas is IL-1ß. The present results suggest that IL-1ß increases the invasive ability of carcinoma cells through activation of NF-B and the resulting MMP-9 expression.

This article has been cited by other articles:

				J. Liu, M. Zhan, J. A.F. Hannay, P. Das, S. V. Bolshakov, D. Kotilingam, D. Yu, A. F. Lazar, R. E. Pollock, and D. Lev Wild-type p53 Inhibits Nuclear Factor-{kappa}B-Induced Matrix Metalloproteinase-9 Promoter Activation: Implications for Soft Tissue Sarcoma Growth and Metastasis Mol. Cancer Res., November 1, 2006; 4(11): 803 - 810. [Abstract] [Full Text] [PDF]

				H. Nakashima, M. Nakamura, H. Yamaguchi, N. Yamanaka, T. Akiyoshi, K. Koga, K. Yamaguchi, M. Tsuneyoshi, M. Tanaka, and M. Katano Nuclear Factor-{kappa}B Contributes to Hedgehog Signaling Pathway Activation through Sonic Hedgehog Induction in Pancreatic Cancer. Cancer Res., July 15, 2006; 66(14): 7041 - 7049. [Abstract] [Full Text] [PDF]