This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tomonaga, T. Articles by Nomura, F. Search for Related Content PubMed PubMed Citation Articles by Tomonaga, T. Articles by Nomura, F.

Identification of Altered Protein Expression and Post-Translational Modifications in Primary Colorectal Cancer by Using Agarose Two-Dimensional Gel Electrophoresis

Departments of ¹ Molecular Diagnosis (F8) and ² Academic Surgery (M9), Graduate School of Medicine, Chiba University, Chiba, Japan, and ³ Laboratory of Biomolecular Dynamics, Department of Physics, Kitasato University School of Science, Kanagawa, Japan

Purpose: Although numerous proteome studies have been performed recently to identify cancer-related changes in protein expression, only a limited display of relatively abundant proteins has been identified. The aim of this study is to identify novel proteins as potential tumor markers in primary colorectal cancer tissues using a high-resolution two-dimensional gel electrophoresis (2-DE).

Experimental Design: 2-DE using an agarose gel for isoelectric focusing was used to compare protein profiling of 10 colorectal cancer tissues and adjacent normal mucosa. Altered expression and post-translational modification of several proteins were examined using Western blot analysis and immunohistochemistry.

Results: Ninety-seven proteins of 107 spots (90.7%) that were differentially expressed between matched normal and tumor tissues were identified by mass spectrometry. Among them, 42 unique proteins (49 spots) significantly increased or decreased in the tumors. They include eukaryotic translation initiation factor 4H, inorganic pyrophosphatase, anterior gradient 2 homologue, aldolase A, and chloride intracellular channel 1, whose elevated expression in tumor tissues was confirmed by Western blot analysis and immunohistochemistry. Interestingly, only isoform 1 of two transcript variants of eukaryotic translation initiation factor 4H was greatly up-regulated in most of the tumor tissues. Moreover, post-translational modifications of the prolyl-4-hydroxylase ß subunit and annexin A2 also were identified.

Conclusions: We identified several novel proteins with altered expression in primary colorectal cancer using agarose 2-DE. This method is a powerful technique with which to search for not only quantitative but also qualitative changes in a biological process of interest and may contribute to the deeper understanding of underlying mechanisms of human cancer.

This article has been cited by other articles:

				S. Takano, A. Togawa, H. Yoshitomi, T. Shida, F. Kimura, H. Shimizu, H. Yoshidome, M. Ohtsuka, A. Kato, T. Tomonaga, et al. Annexin II Overexpression Predicts Rapid Recurrence after Surgery in Pancreatic Cancer Patients Undergoing Gemcitabine-Adjuvant Chemotherapy Ann. Surg. Oncol., November 1, 2008; 15(11): 3157 - 3168. [Abstract] [Full Text] [PDF]

				R. N. Morrison, G. A. Cooper, B. F. Koop, M. L. Rise, A. R. Bridle, M. B. Adams, and B. F. Nowak Transcriptome profiling the gills of amoebic gill disease (AGD)-affected Atlantic salmon (Salmo salar L.): a role for tumor suppressor p53 in AGD pathogenesis? Physiol Genomics, September 14, 2006; 26(1): 15 - 34. [Abstract] [Full Text] [PDF]

				X. Bi, Q. Lin, T. W. Foo, S. Joshi, T. You, H.-M. Shen, C. N. Ong, P. Y. Cheah, K. W. Eu, and C.-L. Hew Proteomic Analysis of Colorectal Cancer Reveals Alterations in Metabolic Pathways: Mechanism of Tumorigenesis Mol. Cell. Proteomics, June 1, 2006; 5(6): 1119 - 1130. [Abstract] [Full Text] [PDF]

				C. F. Basil, Y. Zhao, K. Zavaglia, P. Jin, M. C. Panelli, S. Voiculescu, S. Mandruzzato, H. M. Lee, B. Seliger, R. S. Freedman, et al. Common cancer biomarkers. Cancer Res., March 15, 2006; 66(6): 2953 - 2961. [Abstract] [Full Text] [PDF]

				D. Zhang, L. K. Tai, L. L. Wong, L.-L. Chiu, S. K. Sethi, and E. S. C. Koay Proteomic Study Reveals That Proteins Involved in Metabolic and Detoxification Pathways Are Highly Expressed in HER-2/neu-positive Breast Cancer Mol. Cell. Proteomics, November 1, 2005; 4(11): 1686 - 1696. [Abstract] [Full Text] [PDF]

				M. Roessler, W. Rollinger, S. Palme, M.-L. Hagmann, P. Berndt, A. M. Engel, B. Schneidinger, M. Pfeffer, H. Andres, J. Karl, et al. Identification of Nicotinamide N-Methyltransferase as a Novel Serum Tumor Marker for Colorectal Cancer Clin. Cancer Res., September 15, 2005; 11(18): 6550 - 6557. [Abstract] [Full Text] [PDF]

				J. Shen, M. D. Person, J. Zhu, J. L. Abbruzzese, and D. Li Protein Expression Profiles in Pancreatic Adenocarcinoma Compared with Normal Pancreatic Tissue and Tissue Affected by Pancreatitis as Detected by Two-Dimensional Gel Electrophoresis and Mass Spectrometry Cancer Res., December 15, 2004; 64(24): 9018 - 9026. [Abstract] [Full Text] [PDF]