Clinical Cancer Research Targets Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, W.-P.
Right arrow Articles by Lee, S.-D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, W.-P.
Right arrow Articles by Lee, S.-D.
Clinical Cancer Research Vol. 11, 6431-6441, September 15, 2005
© 2005 American Association for Cancer Research


Human Cancer Biology

The –251T Allele of the Interleukin-8 Promoter Is Associated with Increased Risk of Gastric Carcinoma Featuring Diffuse-Type Histopathology in Chinese Population

Wei-Ping Lee1,4, Dar-In Tai8, Keng-Hsin Lan1,5, Anna Fen-Yau Li2,6, Hou-Ching Hsu1, En-Ju Lin1, Yi-Ping Lin1, Meei-Ling Sheu7, Chung-Pin Li1,6, Full-Young Chang1,6, Yee Chao3,6, Shang-Heu Yen3,6 and Shou-Dong Lee1,6

Authors' Affilliations: 1 Division of Gastroenterology, Department of Medicine, 2 Department of Pathology, and 3 Cancer Center, Taipei Veterans General Hospital; 4 Department and Institute of Biochemistry, 5 Department and Institute of Pharmacology, and 6 School of Medicine, National Yang-Ming University; and 7 Department of Toxicology, National Taiwan University College of Medicine, Taipei, Taiwan; and 8 The Liver Research Unit, Department of Gastroenterology and Hepatology, Chang Gung Memorial Hospital, Taoyuan, Taiwan

Requests for reprints: Wei-Ping Lee, Division of Gastroenterology, Department of Medicine, Taipei Veterans General Hospital, 201 Shi-Pai Road Section 2, Taipei 112, Taiwan. Phone: 886-2-28712121-2017; Fax: 886-2-2874-9425; E-mail: wleemc{at}yahoo.com.

Purpose: Persistent interleukin-8 (IL-8) production contributes to chronic inflammation of the stomach. The proinflammatory IL-1ß polymorphisms, which enhance the cytokine production, are associated with increased risk of gastric cancer. The –251A/T polymorphism of the IL-8 promoter is involved in several human diseases. Particularly, the –251A is associated with decreased risk of colorectal cancer. We aimed to determine whether the –251 allele resulting in high IL-8 expression was associated with increased risk of gastric carcinoma.

Experimental Design: The –251A/T promoters were cloned and analyzed by luciferase assay. Binding of nuclear proteins to the –251A/T promoters was analyzed by electrophoretic mobility shift assay. The –251A/T promoters were differentiated by PCR-RFLP. Comparison of gastric cancer risk between the –251A/T promoters was done by a case-control study.

Results: The –251T allele possessed transcriptional activity 2- to 5-fold stronger than the –251A counterpart. Electrophoretic mobility shift assay showed that the –251A promoter had strong ability to bind to an unknown protein or multiprotein complex. The –251T allele was associated with increased risk of noncardia (Ptrend = 0.012) and cardia (Ptrend = 0.029) carcinomas. Gastric carcinoma patients with the low-risk AA genotype had a tendency to sustain intestinal-type carcinomas ({chi}2 = 6.816; P = 0.033); however, the high-risk –251T allele was associated with >2-fold increased risk of diffuse-type (AA versus AT + TT: odds ratio, 2.52; 95% confidence interval, 1.16-5.49; P = 0.017) and mixed-type (AA versus AT + TT: odds ratio, 2.22; 95% confidence interval, 1.12-4.40; P = 0.019) carcinomas.

Conclusions: The IL-8 –251T allele is significantly associated with increased risk of gastric carcinoma, particularly the diffuse and mixed types in Chinese population.




This article has been cited by other articles:


Home page
J. Nutr.Home page
K.-P. Ko, S. K. Park, L. Y. Cho, J. Gwack, J. J. Yang, A. Shin, C. S. Kim, Y. Kim, D. Kang, S.-H. Chang, et al.
Soybean Product Intake Modifies the Association between Interleukin-10 Genetic Polymorphisms and Gastric Cancer Risk
J. Nutr., May 1, 2009; 139(5): 1008 - 1012.
[Abstract] [Full Text] [PDF]


Home page
Ann OncolHome page
J. B. A. Crusius, F. Canzian, G. Capella, A. S. Pena, G. Pera, N. Sala, A. Agudo, F. Rico, G. Del Giudice, D. Palli, et al.
Cytokine gene polymorphisms and the risk of adenocarcinoma of the stomach in the European prospective investigation into cancer and nutrition (EPIC-EURGAST)
Ann. Onc., November 1, 2008; 19(11): 1894 - 1902.
[Abstract] [Full Text] [PDF]


Home page
Ann OncolHome page
G. Lurje, W. Zhang, A. M. Schultheis, D. Yang, S. Groshen, A. E. Hendifar, H. Husain, M. A. Gordon, F. Nagashima, H. M. Chang, et al.
Polymorphisms in VEGF and IL-8 predict tumor recurrence in stage III colon cancer
Ann. Onc., October 1, 2008; 19(10): 1734 - 1741.
[Abstract] [Full Text] [PDF]


Home page
Br. J. Ophthalmol.Home page
S V Goverdhan, S Ennis, S R Hannan, K C Madhusudhana, A J Cree, A J Luff, and A J Lotery
Interleukin-8 promoter polymorphism -251A/T is a risk factor for age-related macular degeneration
Br. J. Ophthalmol., April 1, 2008; 92(4): 537 - 540.
[Abstract] [Full Text] [PDF]


Home page
IOVSHome page
Y.-Y. Tsai, J.-M. Lin, L. Wan, H.-J. Lin, Y. Tsai, C.-C. Lee, C.-H. Tsai, F.-J. Tsai, and S.-H. Tseng
Interleukin Gene Polymorphisms in Age-Related Macular Degeneration
Invest. Ophthalmol. Vis. Sci., February 1, 2008; 49(2): 693 - 698.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2005 by the American Association for Cancer Research.