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Whole-Genome Allelotyping Identified Distinct Loss-of-Heterozygosity Patterns in Mucinous Ovarian and Appendiceal Carcinomas

Authors' Affiliations: ¹ Department of Obstetrics, Gynecology, and Reproductive Biology, Division of Gynecologic Oncology; ² Department of Pathology, Brigham and Women's Hospital, Harvard Medical School; ³ Department of Biostatistics, Harvard School of Public Health; ⁴ Department of Pathology, Massachusetts General Hospital; ⁵ Gillette Center For Women's Cancer, Dana-Farber Cancer Institute, Boston, Massachusetts; ⁶ Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, University of Texas Southwestern Medical School, Dallas, Texas; and ⁷ Department of Gynecologic Oncology, University of Texas M.D. Anderson Cancer Center, Houston, Texas

Requests for reprints: Colleen M. Feltmate, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115. Phone: 617-732-3344; E-mail: cfeltmate{at}partners.org.

Purpose: Mucinous adenocarcinoma of the ovary is one of the common histologic types of ovarian cancer. Its pathogenesis is largely unknown. In addition, the differential diagnosis of metastatic mucinous carcinomas to the ovaries, particularly those originating from the appendix, remains challenging. The purpose of this study is to identify molecular biomarkers for mucinous ovarian adenocarcinoma and compare them with those of appendiceal origin.

Experimental Design: Genome-wide loss-of-heterozygosity (LOH) analysis was done on DNA isolated from 28 microdissected primary mucinous ovarian carcinomas and five appendiceal adenocarcinomas. Markers from high-loss regions were selected for further analysis on a total of 32 ovarian and 14 appendiceal cancers.

Results: High levels of LOH rates (>40%) were detected on chromosome arms 9p, 17p, and 21q in mucinous ovarian carcinoma cases. The frequency of allelic loss was similar between high-grade and low-grade mucinous ovarian carcinoma cases but was significantly higher in ovarian versus appendiceal cases. In addition, LOH rates on five chromosomal loci were statistically different between ovarian and appendiceal carcinomas.

Conclusion: A high frequency of LOH can be found in mucinous ovarian adenocarcinomas independent of grade. Despite histologic similarities between mucinous ovarian carcinomas and metastatic appendiceal carcinomas, they have distinct LOH profiles, which may be used for distinguishing the two diseases.

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