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Serum Free Light Chain Analysis and Urine Immunofixation Electrophoresis in Patients with Multiple Myeloma

Authors' Affiliations: ¹ Department of Internal Medicine (Cancer Research), West German Cancer Center, University Hospital of Essen Medical School, Essen, Germany; ² Division of Immunology and Infection, University of Birmingham; and ³ The Binding Site Ltd., Birmingham, United Kingdom

Requests for reprints: Mohammad R. Nowrousian, Department of Internal Medicine (Cancer Research), West German Cancer Center, University Hospital of Essen Medical School, Hufelandstrasse 55, 45122 Essen, Germany. Phone: 49-201-723-3127; Fax: 49-201-723-5984; E-mail: nowrousian{at}uni-essen.de.

Purpose: Retrospective studies have shown that immunoassays measuring free light chains (FLC) in serum are useful for diagnosis and monitoring of multiple myeloma. This study prospectively evaluates the use of FLC assays and, for the first time, investigates the relationship between serum FLC concentrations and the presence and detectability of Bence Jones (BJ) proteins in the urine.

Patients and Methods: Three hundred seventy-eight paired samples of serum and urine were tested from 82 patients during the course of their disease. The sensitivities of serum FLC analysis and urine immunofixation electrophoresis (IFE) in detecting monoclonal FLC were compared. Serum FLC concentrations required for producing BJ proteins detected by IFE were determined.

Results: Abnormal FLC were present in 54% of serum samples compared with 25% by urine tests. In abnormal serum samples for or , the sensitivity of IFE to detect the respective BJ proteins in urine were 51% and 35% and the median serum FLC concentrations required to produce detectable BJ proteins were 113 and 278 mg/L. Renal excretions of monoclonal FLC increased with serum concentrations, but excretions significantly decreased at high serum concentrations combined with renal dysfunction.

Conclusion: Serum FLC assays are significantly more sensitive for detecting monoclonal FLC than urine IFE analysis. They also have the advantage of FLC quantification and are more reliable for monitoring disease course and response to treatment.

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