| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Human Cancer Biology |
1 Service d'Histologie-Biologie Tumorale and 2 Service d'Anatomie Pathologique, Unité Propre de Recherche de l'Enseignement Supérieur EA 3499, Université Paris 6, Hôpital Tenon, Paris, France and 3 Unité Mixte de Recherche-Centre National de la Recherche Scientifique 6185, Université de Caen, Caen, France
Requests for reprints: Patrice Callard, Service d'Anatomie-Pathologique and Unité Propre de Recherche de l'Enseignement Supé rieur EA 3499, Université Paris 6, Hôpital Tenon, 4 rue de la Chine, 75970 Paris cedex 20, France. Phone: 33-1-56-01-70-13; Fax: 33-1-56-01-78-76; E-mail: patrice.callard{at}tnn.ap-hop-paris.fr.
Purpose: Expression of erythropoietin (Epo) and its receptor (Epo-R) has been shown in various normal and neoplastic nonhematopoietic tissues. This study, in non–small cell lung carcinoma, was designed to investigate the previously unreported expression of Epo and Epo-R as well as hypoxia-inducible factor-1
(HIF-1), which is known to control Epo expression.
Experimental Design: Samples from lung squamous cell carcinomas (n = 17) and adenocarcinomas (n = 12) were obtained from patients undergoing curative surgery. mRNA transcripts of Epo, Epo-R, soluble Epo-R (sEpo-R), HIF-1, and factor inhibiting HIF-1 (FIH-1) were evaluated by reverse transcription-PCR, whereas localization of Epo, Epo-R, and HIF-1 was assessed by immunohistochemistry.
Results: Epo, Epo-R, sEpo-R, HIF-1, and FIH-1 transcripts were detected by reverse transcription-PCR in all samples tested, but with heterogeneous levels of expression for Epo, Epo-R, and sEpo-R. Coordinated levels of mRNA were observed for HIF-1 and FIH-1.
Epo was detected in carcinomatous cells by immunohistochemistry in 50% of samples and Epo-R was detected in 96% of samples. Co-expression of Epo and Epo-R was observed on contiguous sections from 50% of tumors. HIF-1 was immunolocalized in 80% of non–small cell lung carcinomas.
Conclusion: Epo-R was expressed in almost all samples and Epo was expressed in one half of samples on immunohistochemistry and in 100% of samples by mRNA detection, suggesting a potential paracrine and/or autocrine role of endogenous Epo in non–small cell lung carcinoma. The detection of stabilized HIF-1 suggests a possible role in Epo expression. Moreover, in the light of these results, the potential interactions between therapeutic recombinant Epo and the putative neoplastic Epo/Epo-R signaling pathways must be considered.
This article has been cited by other articles:
|
|
 |
|
  P. Saintigny, B. Besse, J.-C. Soria, J.-F. Bernaudin, and P. Callard Does Erythropoietin Promote Tumor Growth? Clin. Cancer Res., March 15, 2008; 14(6): 1920 - 1921. [Full Text] [PDF]
|
|
|
|
|
|
P. Saintigny, B. Besse, P. Callard, A.-C. Vergnaud, S. Czernichow, M. Colombat, P. Girard, P. Validire, J.-L. Breau, J.-F. Bernaudin, et al. Erythropoietin and Erythropoietin Receptor Coexpression Is Associated with Poor Survival in Stage I Non Small Cell Lung Cancer Clin. Cancer Res., August 15, 2007; 13(16): 4825 - 4831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Sytkowski Does Erythropoietin Have a Dark Side? Epo Signaling and Cancer Cells Sci. Signal., July 17, 2007; 2007(395): pe38 - pe38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kokhaei, A. O. Abdalla, L. Hansson, E. Mikaelsson, M. Kubbies, A. Haselbeck, H. Jernberg-Wiklund, H. Mellstedt, and A. Osterborg Expression of Erythropoietin Receptor and In vitro Functional Effects of Epoetins in B-Cell Malignancies Clin. Cancer Res., June 15, 2007; 13(12): 3536 - 3544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zeuner, M. Signore, D. Martinetti, M. Bartucci, C. Peschle, and R. De Maria Chemotherapy-Induced Thrombocytopenia Derives from the Selective Death of Megakaryocyte Progenitors and Can Be Rescued by Stem Cell Factor Cancer Res., May 15, 2007; 67(10): 4767 - 4773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ferretti, L. Bruni, V. Dangles-Marie, A.P. Pecking, and D. Bellet Molecular circuits shared by placental and cancer cells, and their implications in the proliferative, invasive and migratory capacities of trophoblasts Hum. Reprod. Update, March 1, 2007; 13(2): 121 - 141. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. M. Brown, P. Maxwell, A. N.J. Graham, A. Yakkundi, E. A. Dunlop, Z. Shi, P. G. Johnston, and T. R.J. Lappin Erythropoietin Receptor Expression in Non-Small Cell Lung Carcinoma: A Question of Antibody Specificity Stem Cells, March 1, 2007; 25(3): 718 - 722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Dunlop, A. P. Maxwell, and T. R.J. Lappin Impaired Downregulation Following Erythropoietin Receptor Activation in Non-Small Cell Lung Carcinoma Stem Cells, February 1, 2007; 25(2): 380 - 384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Leo, L.-C. Horn, C. Rauscher, B. Hentschel, A. Liebmann, G. Hildebrandt, and M. Hockel Expression of Erythropoietin and Erythropoietin Receptor in Cervical Cancer and Relationship to Survival, Hypoxia, and Apoptosis Clin. Cancer Res., December 1, 2006; 12(23): 6894 - 6900. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Henke, D. Mattern, M. Pepe, C. Bezay, C. Weissenberger, M. Werner, and F. Pajonk Do Erythropoietin Receptors on Cancer Cells Explain Unexpected Clinical Findings? J. Clin. Oncol., October 10, 2006; 24(29): 4708 - 4713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Cai, K. Lan, S. C. Verma, H. Si, D. Lin, and E. S. Robertson Kaposi's Sarcoma-Associated Herpesvirus Latent Protein LANA Interacts with HIF-1{alpha} To Upregulate RTA Expression during Hypoxia: Latency Control under Low Oxygen Conditions. J. Virol., August 1, 2006; 80(16): 7965 - 7975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Henke, A. Verma, G. Acs, S. Elliott, L. Busse, C. Spahr, and A. M. Sinclair Erythropoietin receptors on cancer cells: exciting perspectives, difficult to appreciate Blood, August 1, 2006; 108(3): 1107 - 1109. [Full Text] [PDF]
|
|
|
|
|
|
S. Elliott, L. Busse, M. B. Bass, H. Lu, I. Sarosi, A. M. Sinclair, C. Spahr, M. Um, G. Van, and C. G. Begley Anti-Epo receptor antibodies do not predict Epo receptor expression Blood, March 1, 2006; 107(5): 1892 - 1895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Hardee, M. O. Arcasoy, K. L. Blackwell, J. P. Kirkpatrick, and M. W. Dewhirst Erythropoietin Biology in Cancer Clin. Cancer Res., January 15, 2006; 12(2): 332 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Winter, K. A. Shah, L. Campo, H. Turley, R. Leek, R. J. Corbridge, G. J. Cox, and A. L. Harris Relation of Erythropoietin and Erythropoietin Receptor Expression to Hypoxia and Anemia in Head and Neck Squamous Cell Carcinoma Clin. Cancer Res., November 1, 2005; 11(21): 7614 - 7620. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Cell Growth & Differentiation |
