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Array Comparative Genomic Hybridization Identifies Genetic Subgroups in Grade 4 Human Astrocytoma

Authors' Affiliations: ¹ Brain Tumor Research Center, Department of Neurosurgery, ² Department of Laboratory Medicine, ³ Cancer Research Institute, and ⁴ Comprehensive Cancer Center, University of California, San Francisco; San Francisco, California

Requests for reprints: Anjan Misra, Brain Tumor Research Center, Department of Neurosurgery, University of California, San Francisco, 2340 Sutter Sreet, San Francisco, CA. Phone: 415-476-0633; Fax: 415-476-8218; E-mail: amisra{at}cc.ucsf.edu.

Alterations of DNA copy number are believed to be important indicators of tumor progression in human astrocytoma. We used an array of bacterial artificial chromosomes to map relative DNA copy number in 50 primary glioblastoma multiforme tumors at 1.4-Mb resolution. We identified 33 candidate sites for amplification and homozygous deletion in these tumors. We identified three major genetic subgroups within these glioblastoma multiforme tumors: tumors with chromosome 7 gain and chromosome 10 loss, tumors with only chromosome 10 loss in the absence of chromosome 7 gain, and tumors without copy number change in chromosomes 7 or 10. The significance of these genetic groups to therapeutics needs further study.

Key Words: Brain tumor • Glioma • Glioblastoma Multiforme • DNA array • Genetic classification

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