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Intratumoral Therapy with IL13-PE38 Results in Effective CTL-Mediated Suppression of IL-13R2-Expressing Contralateral Tumors

Authors' Affiliations: ¹ Tumor Vaccines and Biotechnology Branch, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville Pike, Rockville; ² Vaccine Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland; and ³ Department of Advanced Clinical Science and Therapeutics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

Requests for reprints: Koji Kawakami, Department of Pharmacoepidemiology, Graduate School of Medicine and Public Health, Kyoto University, Yoshida Konoecho, Sakyoku, Kyoto 606-8501, Japan. Phone: 81-75-753-4459; Fax: 81-75-753-4469; E-mail: kawakami-k{at}umin.ac.jp.

Purpose: IL13-PE38, a targeted cytotoxin comprised of interleukin 13 (IL-13) and a mutated form of Pseudomonas exotoxin, induces specific killing of tumor cells expressing abundant levels of the IL-13R2 chain. We hypothesized that tumor cells killed by the cytotoxin may release antigens and/or apoptotic bodies when cells are dying, which then induce adoptive immunity, and that the PE38 portion of IL13-PE38 may act as a stimulant for the induction of a CTL response.

Experimental Design: To test this hypothesis, we established D5 melanoma tumors with or without expression of the IL-13R2 chain in both flanks of C57BL/6 mice, and then IL13-PE38 was injected in the right flank tumors only.

Results and Conclusions: When animals with IL-13R2-expressing D5 tumor (right) were injected with IL13-PE38, right flank tumors expressing the IL-13R2 chain not only showed dramatic regression but contralateral tumors (left flank) also showed tumor regression. Cell depletion experiments in tumor-bearing animals indicated that both CD8⁺ and CD4⁺ T cells contribute to the regression of contralateral tumors through CTL activation in the periphery and cellular infiltration into tumors. In addition, intratumoral treatment into s.c. tumors of mice bearing metastatic lung tumors with IL13-PE38 showed not only the reduction of treated s.c. tumor but also the reduction of lung metastasis. Thus, IL13-PE38 mediates an antitumor effect not only directly but also indirectly by inducing a host CD8⁺ T cell immune response. Accordingly, targeted cytotoxins may be used to treat local disease even if they cannot be administered systemically, and yet may still induce a reasonable systemic antitumor response.

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