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DNA Repair Pathway Profiling and Microsatellite Instability in Colorectal Cancer

Authors' Affiliations: Departments of ¹ Medicine, ² Surgery, ³ Genetics, ⁴ Pathology & Immunology, and ⁵ Molecular Biology & Pharmacology, Washington University School of Medicine and ⁶ Siteman Cancer Center, Saint Louis, Missouri

Requests for reprints: Howard L. McLeod, Washington University School of Medicine, 660 South Euclid Avenue-Campus Box 8069, Saint Louis, MO 63110-1093. Phone: 314-747-5183; Fax: 314-362-3764; E-mail: hmcleod{at}im.wustl.edu.

Background: The ability to maintain DNA integrity is a critical cellular function. DNA repair is conducted by distinct pathways of genes, many of which are thought to be altered in colorectal cancer. However, there has been little characterization of these pathways in colorectal cancer.

Method: By using the TaqMan real-time quantitative PCR, RNA expression profiling of 20 DNA repair pathway genes was done in matched tumor and normal tissues from 52 patients with Dukes' C colorectal cancer.

Results: The relative mRNA expression level across the 20 DNA repair pathway genes varied considerably, and the individual variability was also quite large, with an 85.4 median fold change in the tumor tissue genes and a 127.2 median fold change in the normal tissue genes. Tumor-normal differential expression was found in 13 of 20 DNA repair pathway genes (only XPA had a lower RNA level in the tumor samples; the other 12 genes had significantly higher tumor levels, all P < 0.01). Coordinated expression of ERCC6, HMG1, MSH2, and POLB (R_S 0.60) was observed in the tumor tissues (all P < 0.001). Apoptosis index was not correlated with expression of the 20 DNA repair pathway genes. MLH1 and XRCC1 RNA expression was correlated with microsatellite instability status (P = 0.045 and 0.020, respectively). An inverse correlation was found between tumor MLH1 RNA expression and MLH1 DNA methylation (P = 0.003).

Conclusion: Our study provides an initial characterization of the DNA repair pathways for understanding the cellular DNA damage/repair system in human colorectal cancer.

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