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Decreased Mitochondrial DNA Content in Posttreatment Salivary Rinses from Head and Neck Cancer Patients

Authors' Affiliations: Departments of ¹ Otolaryngology-Head and Neck Surgery, Head and Neck Cancer Division; ² Oncology; ³ Oncology Biostatistics; ⁴ Pathology; and ⁵ Epidemiology, Johns Hopkins Medical Institutions, Baltimore, Maryland

Requests for reprints: Joseph A. Califano, Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins Medical Institutions, 6th Floor, 601 North Caroline Street, Baltimore, MD 21287-0910. Phone: 410-502-5153; Fax: 410-614-1411; E-mail: jcalifa{at}jhmi.edu.

Purpose and Experimental Design: Alterations in mitochondrial DNA (mtDNA) sequence and content have been described in human tissues and tumors in association with smoking exposure. We did quantitative PCR analysis of cytochrome c oxidase (Cox) I and Cox II genes to measure changes in mtDNA content in pretreatment and posttreatment salivary rinses obtained from 76 patients undergoing surgical resection for primary head and neck squamous cell carcinoma. We also examined the relationship between changes in mtDNA content and postoperative radiation therapy, smoking exposure, alcohol intake, and other clinical characteristics.

Results: Overall, mtDNA content in posttreatment saliva was significantly decreased. The mean change for Cox I was –0.21 [95% confidence interval (95% CI), –0.44 to 0.01, P = 0.06] and for Cox II was –0.31 (95% CI, –0.55 to –0.08, P = 0.01). Patients in the radiation therapy group exhibited a significant decrease compared with the nonradiated group (P = 0.03 for Cox I; P = 0.05 for Cox II). In addition, significant decreases in Cox I (–0.71; 95% CI, –1.17 to –0.25, P = 0.005) and Cox II (–0.65; 95% CI, –1.17 to –0.13, P = 0.02) were found in never-smoking patients but not in former or current smokers.

Conclusion: Our data suggest that salivary mtDNA content is decreased in never smokers and in response to radiation therapy after primary surgical resection.