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Delineation, Functional Validation, and Bioinformatic Evaluation of Gene Expression in Thyroid Follicular Carcinomas with the PAX8-PPARG Translocation

Authors' Affiliations: Departments of ¹ Pathology, ² Pediatrics, and ³ Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan; ⁴ Cancer Genetics Unit, Kolling Institute of Medical Research, University of Sydney, New South Wales, Australia; ⁵ Department of Pathology, University of Cincinnati College of Medicine, Cincinnati, Ohio; and ⁶ Fred Hutchinson Cancer Research Center, Seattle, Washington

Requests for reprints: Thomas J. Giordano, Department of Pathology, UH 2G332/0054, Ann Arbor, MI 48109-0054. Phone: 734-936-6776; Fax: 734-763-4095; E-mail: Giordano{at}umich.edu.

A subset of follicular thyroid carcinomas contains a balanced translocation, t(2;3)(q13;p25), that results in fusion of the paired box gene 8 (PAX8) and peroxisome proliferator-activated receptor (PPARG) genes with concomitant expression of a PAX8-PPAR fusion protein, PPFP. PPFP is thought to contribute to neoplasia through a mechanism in which it acts as a dominant-negative inhibitor of wild-type PPAR. To better understand this type of follicular carcinoma, we generated global gene expression profiles using DNA microarrays of a cohort of follicular carcinomas along with other common thyroid tumors and used the data to derive a gene expression profile characteristic of PPFP-positive tumors. Transient transfection assays using promoters of four genes whose expression was highly associated with the translocation showed that each can be activated by PPFP. PPFP had unique transcriptional activities when compared with PAX8 or PPAR, although it had the potential to function in ways qualitatively similar to PAX8 or PPAR depending on the promoter and cellular environment. Bioinformatics analyses revealed that genes with increased expression in PPFP-positive follicular carcinomas include known PPAR target genes; genes involved in fatty acid, amino acid, and carbohydrate metabolism; micro-RNA target genes; and genes on chromosome 3p. These results have implications for the neoplastic mechanism of these follicular carcinomas.

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