This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Liu, T. Articles by Yao, K. Search for Related Content PubMed PubMed Citation Articles by Liu, T. Articles by Yao, K.

An Imageable Metastatic Treatment Model of Nasopharyngeal Carcinoma

Authors' Affiliations: ¹ Cancer Institute, Nanfang Hospital, Southern Medical University, Key Laboratory of Transcriptomics and Proteomics of Human Diseases; ² Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Guangzhou, China; ³ AntiCancer, Inc.; and ⁴ Department of Surgery, University of California–San Diego, San Diego, California

Requests for reprints: Robert M. Hoffman, AntiCancer, Inc., San Diego, CA 92111. Phone: 1-858-654-2555; Fax: 1-858-268-4175; E-mail: all{at}anticancer.com.

Purpose: Nasopharyngeal carcinoma is highly prevalent in southern China and is often resistant to current treatment options.

Experimental Design: Clinically relevant mouse models are necessary for further understanding and drug discovery in this disease. Two nasopharyngeal carcinoma cell lines, stably expressing green fluorescent protein (GFP), 5-8F-GFP and 6-10B-GFP, were established. The cells were orthotopically injected into the nasopharynx or ectopically into the subcutis of nude mice. Whole-body fluorescence imaging was used to monitor the growth of the primary tumor as well as angiogenesis and metastasis.

Results: The metastatic behavior of 5-8F and 6-10B were distinct in the orthotopic model. Orthotopic implantation of highly metastatic 5-8F cells resulted in brain invasion, cervical lymph node metastases, and pulmonary metastases similar to what is often observed in patients. Cell line 6-10B was less metastatic, which occasionally resulted in pulmonary metastasis. GFP enabled imaging of micrometastasis. Neither 5-8F nor 6-10B were metastatic in the s.c. site. These results indicated that, in addition to the cancer cell type, the host microenvironment was critical for metastasis to occur consistent with the "seed-and-soil" hypothesis. 5-8F was highly sensitive to 5-fluorouracil (5-FU), whereas 6-10B was moderately sensitive.

Conclusions: The imageable orthotopic model should play a critical role in elucidating the mechanisms involved in the growth, progression, metastasis, and angiogenesis of nasopharyngeal carcinoma and for evaluation of novel compounds with potential efficacy.