This Article Full Text Full Text (PDF) Supplementary Data Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Geerts, D. Articles by Bachmann, A. S. Search for Related Content PubMed PubMed Citation Articles by Geerts, D. Articles by Bachmann, A. S.

Expression of Prenylated Rab Acceptor 1 Domain Family, Member 2 (PRAF2) in Neuroblastoma: Correlation with Clinical Features, Cellular Localization, and Cerulenin-Mediated Apoptosis Regulation

Authors' Affiliations: ¹ Cancer Research Center of Hawaii; ² Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, Hawaii and ³ Department of Human Genetics, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands

Requests for reprints: André S. Bachmann, Cancer Research Center of Hawaii, Natural Products and Cancer Biology Program, University of Hawaii at Manoa, 1236 Lauhala Street, Honolulu, HI 96813. Phone: 808-586-2962; Fax: 808-586-2970; E-mail: abachmann{at}crch.hawaii.edu.

Purpose: Prenylated Rab acceptor 1 domain family, member 2 (PRAF2) is a novel 19-kDa protein that has recently been implicated in human cancer. In the present study, we analyzed for the first time PRAF2 mRNA expression in a large set of human tumors. The high expression in neuroblastic tumors prompted us to analyze PRAF2 expression correlations with genetic and clinical features of these tumors. In addition, we determined the localization of PRAF2 protein in neuroblastoma cells and studied its regulation in apoptosis.

Experimental Design: Affymetrix microarray analysis was done with a set of 41 different tumor types (1,426 samples) in the public domain, a set of three different neuroblastic tumor types (110 samples), and a panel of 25 neuroblastoma cell lines. The subcellular localization of endogenous PRAF2 in neuroblastoma cells was identified by immunofluorescence microscopy and apoptosis detected by Annexin V staining and poly(ADP-ribose) polymerase cleavage.

Results: PRAF2 mRNA was detected in 970 of 1,426 samples in the public data set. All 110 neuroblastic tumors expressed PRAF2 at higher levels than any other tumor examined. Importantly, PRAF2 expression levels significantly correlated with the following clinical features: patient age at diagnosis (P = 6.19 x 10^–5), survival (P = 1.32 x 10^–3), International Neuroblastoma Staging System stage (P = 2.86 x 10^–4), and MYCN amplification (P = 3.74 x 10^–3). PRAF2 localized in bright cytoplasmic punctae and protein levels increased in neuroblastoma cells that underwent cerulenin-induced apoptosis.

Conclusions: Elevated PRAF2 expression levels correlated with unfavorable genetic and clinical features, suggesting PRAF2 as a candidate prognostic marker of neuroblastoma.

This article has been cited by other articles:

				C. L. Alvim Kamei, J. Boruc, K. Vandepoele, H. Van den Daele, S. Maes, E. Russinova, D. Inze, and L. De Veylder The PRA1 Gene Family in Arabidopsis Plant Physiology, August 1, 2008; 147(4): 1735 - 1749. [Abstract] [Full Text] [PDF]