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Proteomics of Canine Lymphoma Identifies Potential Cancer-Specific Protein Markers

Authors' Affiliations: ¹ Department of Veterinary Medicine and Surgery, ² Division of Biological Sciences, ³ Proteomic Center, Christopher S. Bond Life Sciences Center, ⁴ Department of Veterinary Pathobiology, Veterinary Medical Diagnostic Laboratory, and ⁵ School of Medicine, University of Missouri, Columbia, Missouri

Requests for reprints: Dudley L. McCaw, Department of Veterinary Medicine and Surgery, University of Missouri, Clydesdale Hall, 379 East Campus Drive, Columbia, MO 65211. Phone: 573-882-7821; Fax: 573-884-5444; E-mail: McCawD{at}missouri.edu.

Purpose: Early diagnosis of cancer is crucial for the success of treatment of the disease, and there is a need for markers whose differential expression between disease and normal tissue could be used as a diagnostic tool. Spontaneously occurring malignancies in pets provide a logical tool for translational research for human oncology. Lymphoma, one of the most common neoplasms in dogs, is similar to human non–Hodgkin's lymphoma and could serve as an experimental model system.

Experimental Design: Thirteen lymph nodes from normal dogs and 11 lymph nodes from dogs with B-cell lymphoma were subjected to proteomic analysis using two-dimensional PAGE separation and matrix-assisted laser desorption/ionization time-of-flight analysis.

Results: A total of 93 differentially expressed spots was subjected to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry analysis, and several proteins that showed differential expression were identified. Of these, prolidase (proline dipeptidase), triosephosphate isomerase, and glutathione S-transferase were down-regulated in lymphoma samples, whereas macrophage capping protein was up-regulated in the lymphoma samples.

Conclusions: These proteins represent potential markers for the diagnosis of lymphoma and should be further investigated in human samples for validation of their utility as diagnostic markers.