| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Clinical Cancer Research, Vol 2, Issue 5 873-876, Copyright © 1996 by American Association for Cancer Research
ARTICLES |
M Ward, P Pioli, J Ayello, R Reiss, G Urzi, C Richardson, C Hesdorffer and A Bank
Columbia University, College of Physicians and Surgeons, Department of Genetics and Development and Department of Medicine, New York, New York 10032, USA.
The multiple drug resistance (MDR) gene P-glycoprotein product is a transmembrane efflux pump that prevents toxicity of a variety of chemotherapeutic agents, including the anthracyclines, Vinca alkaloids, podophyllins, and taxol. The bone marrow toxicity of these drugs is due to the low or absent expression of MDR in marrow cells. Transfer and expression of the human MDR gene into bone marrow progenitors should prevent this toxicity. We report here the efficient transfer and expression of the MDR gene by retroviral-mediated gene transfer into CD34(+) cells isolated from peripheral blood progenitor cells (PBPCs), comparable to that obtained using bone marrow-derived progenitors. Optimal MDR transduction of these PBPC-derived cells requires exposure to growth factors and a period of preincubation. In addition, we demonstrate that we can transduce up to 100% of progenitor cells derived from PBPCs and can protect up to 25% of these progenitors from a dose of taxol toxic to untransduced controls.
This article has been cited by other articles:
|
|
 |
|
  C. Konetschny, G. W. Holzer, and F. G. Falkner Retroviral Vectors Produced in the Cytoplasmic Vaccinia Virus System Transduce Intron-Containing Genes J. Virol., February 1, 2002; 76(3): 1236 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schiedlmeier, K. Kuhlcke, H. G. Eckert, C. Baum, W. J. Zeller, and S. Fruehauf Quantitative assessment of retroviral transfer of the human multidrug resistance 1 gene to human mobilized peripheral blood progenitor cells engrafted in nonobese diabetic/severe combined immunodeficient mice Blood, February 15, 2000; 95(4): 1237 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Licht, F. Herrmann, M.M. Gottesman, and I. Pastan In Vivo Drug-Selectable Genes: A New Concept in Gene Therapy Stem Cells, March 1, 1997; 15(2): 104 - 111. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. S. Reese, O. N. Koc, K. M. Lee, L. Liu, J. A. Allay, W. P. Phillips Jr., and S. L. Gerson Retroviral transduction of a mutant methylguanine DNA methyltransferase gene into human CD34 cells confers resistance to O6-benzylguanine plus 1,3-bis(2-chloroethyl)-1-nitrosourea PNAS, November 26, 1996; 93(24): 14088 - 14093. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
