Clinical Cancer Research, Vol 3, Issue 10 1707-1711, Copyright © 1997 by American Association for Cancer Research

ARTICLES

Intracellular levels of SGP-2 (Clusterin) correlate with tumor grade in prostate cancer

J Steinberg, R Oyasu, S Lang, S Sintich, A Rademaker, C Lee, JM Kozlowski and JA Sensibar
Departments of Urology, Pathology, and Preventive Medicine, Northwestern University Medical School, Chicago, Illinois 60611, USA.

Our previous observations in LNCaP cells in vitro demonstrated an association between apoptotic cell death resistance and SGP-2 (Clusterin) overexpression. Accordingly, we hypothesized that high levels of cellular SGP-2 would aid in identifying biologically aggressive prostate cancer cells with unique survival advantages. To test this hypothesis, 40 archival radical prostatectomy and/or biopsy specimens of varying grades of prostate cancer were subjected to immunohistochemical SGP-2 staining. The resulting epithelial stains were quantified subjectively on a scale of 1-3 by four independent observers. Benign prostatic epithelial cells from young donors served as controls and showed a consistently weak staining intensity. In contrast, prostate cancer specimens showed varying degrees of staining intensity that correlated with a Gleason pattern (P = 0.006). This correlation supports the hypothesis that protection from apoptotic death may account, in part, for biologically aggressive tumor behavior.

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