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Isolation of Human Lymphocyte Antigens Class I-restricted Cytotoxic T Lymphocytes against Autologous Myeloma Cells¹

Institut National de la Santé et de la Recherche Médicale U463 [C. P-D., G. J., J-L. H., H. V., R. B.] and Laboratoire d’Hématologie, [R. B.] Institut de Biologie, 44093 Nantes cedex 01, and Service d’Hématologie Clinique, Hôtel Dieu [J-L. H.], 44035 Nantes cedex 01, France

Peripheral blood T cells from a patient with multiple myeloma in complete remission were selected in vitro against an autologous myeloma cell line (SBN-1), using a protocol designed for the selection of relatively rare precursor cytotoxic T cells (pCTL). Delayed addition (2 weeks) of interleukin 2 induced T-cell proliferation, and a bulk culture (T-cell line) was obtained 2 days later. This T-cell line displayed cytotoxicity against SBN-1. A CD8⁺ CD4^- cytotoxic T-cell clone (CT5) was then obtained that recognized SBN-1 but not autologous EBV+ B-lymphoblastoid cells, autologous T PHA-blasts, or Daudi, Raji, K562, and 11 allogeneic myeloma cell lines. Moreover, CT5 cytotoxic activity against SBN-1 was blocked by monoclonal antibodies recognizing human lymphocyte antigen class I molecules. This seems to be the first demonstration of myeloma-specific pCTL in peripheral blood T cells of patients with multiple myeloma.

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