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Human Herpesvirus 8 Open Reading Frame 26 and Open Reading Frame 65 Sequences from Multiple Myeloma Patients: A Shared Pattern Not Found in Kaposi’s Sarcoma or Primary Effusion Lymphoma¹

Division of Hematology and Oncology, Cedars-Sinai Medical Center [H. J. M., N. N. S-S., R. A. V., M. K., A. M., K. P., D. B., J. R. B.], Division of Hematology and Oncology, Veterans Affairs Greater Los Angeles Health Care System [M. P., C. H. W., J. Z., L. Z., G. C.], Division of Pathology [J. W. S.], University of California Los Angeles School of Medicine, and Jonsson Comprehensive Cancer Center [H. J. M., N. N. S-S., R. A. V., M. K., A. M., M. P., K. P., D. B., C. H. W., J. Z., L. Z., G. C., J. R. B.], Los Angeles, California 90048; Department of Hematology, Ibni Sina Hospital, Sihhiye Ankara, Turkey [M. B.]; and BIS Laboratories, Reseda, California [T. J. M.]

ABSTRACT

Human herpesvirus 8 (HHV-8), also known as Kaposi’s sarcoma-associated herpesvirus, has been implicated in the pathogenesis of Kaposi’s sarcoma (KS), primary effusion lymphoma (PEL), multicentric Castleman’s disease, and recently multiple myeloma (MM). DNA sequence analyses of HHV-8 suggest that multiple HHV-8 strains exist. We extracted DNA from 24 patients with MM and 3 patients with monoclonal gammopathy of undetermined significance and compared HHV-8 open reading frames (ORFs) 26 and 65 sequences with those derived from patients with KS, PEL, and two HHV-8-positive PEL cell lines KS-1 and BC-1. ORF26 sequence data suggest that MM patients are consistently carriers of HHV-8 strain subtype C3. All MM patients also consistently revealed either a single bp deletion or substitution at position 112197 in ORF65. This unique alteration is not present in patients with KS or PEL or in PEL cell lines. It occurs in the portion of ORF65 that is known to be responsible for a serological response to HHV-8.

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