This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ghanem, M. A. Articles by Van Steenbrugge, G. J. Search for Related Content PubMed PubMed Citation Articles by Ghanem, M. A. Articles by Van Steenbrugge, G. J.

Expression and Prognostic Value of Wilms’ Tumor 1 and Early Growth Response 1 Proteins in Nephroblastoma

Departments of Pediatric Urology [M. A. G., M. H. A. O., M. A. N., R. M. N., G. J. V. S.] and Pathology [T. H. V. d. K., J. C. D. H.], Josephine Nefkens Institute, and The Netherlands Institute for Health Sciences [M. K. S.], Erasmus University, 3000 DR Rotterdam, the Netherlands; Department of Pediatric Oncology, University Hospital, 3015 GD Rotterdam, the Netherlands [M. M. V. d. H.]; and Urology Department, Menoufiya University, Shebin El-Kom, Egypt [S. M. N.]

ABSTRACT

Wilms’ tumor is one of the most common solid tumors of children. The protein product of the tumor-suppressor gene, Wilms’ tumor 1 (WT-1), binds to the same DNA sequences as the protein product of the early growth response 1 (EGR-1) gene. There is experimental evidence that EGR-1 is involved in controlling cell growth. The expression of both genes in Wilms’ tumor was studied by others, mainly at the mRNA level. The present study evaluates the prognostic value of WT-1 and EGR-1 in 61 Wilms’ tumors of chemotherapeutically treated patients at the protein level, using an immunohistochemical approach. WT-1 was expressed in normal kidney tissues and in the blastemal and epithelial component of Wilms’ tumor, whereas stromal tissue was negative. EGR-1 was expressed in normal kidney tissues and in the three main cell types of Wilms’ tumor. In 59 and 56% of Wilms’ tumor, the blastemal cells stained for WT-1 and EGR-1, respectively. The blastemal expression of WT-1 and EGR-1 and the epithelial expression of WT-1 were statistically significantly correlated with clinical stage. WT-1 immunoreactivity correlated with EGR-1 expression. Univariate analysis showed that blastemal WT-1 and EGR-1 expression were indicative for clinical progression and tumor-specific survival, whereas epithelial staining was of no prognostic value. Multivariate analysis showed that blastemal WT-1 expression is an independent prognostic marker for clinical progression other than stage. We conclude that a relationship exists between WT-1 and EGR-1 expression in clinical nephroblastomas. Blastemal WT-1 and EGR-1 expression is related to prognosis.

This article has been cited by other articles:

				C. Jones, K. Pritchard-Jones, M. A. Ghanem, T. H. Van der Kwast, R. M. Nijman, and G. J. van Steenbrugge MIB-1 and p27Kip1 Expression in Nephroblastoma Clin. Cancer Res., November 15, 2004; 10(22): 7785 - 7786. [Full Text] [PDF]

				W. M. Kong, N. M. Martin, K. L. Smith, J. V. Gardiner, I. P. Connoley, D. A. Stephens, W. S. Dhillo, M. A. Ghatei, C. J. Small, and S. R. Bloom Triiodothyronine Stimulates Food Intake via the Hypothalamic Ventromedial Nucleus Independent of Changes in Energy Expenditure Endocrinology, November 1, 2004; 145(11): 5252 - 5258. [Abstract] [Full Text] [PDF]

				M. Takahashi, X. J. Yang, T. T. Lavery, K. A. Furge, B. O. Williams, M. Tretiakova, A. Montag, N. J. Vogelzang, G. G. Re, A. J. Garvin, et al. Gene Expression Profiling of Favorable Histology Wilms Tumors and Its Correlation with Clinical Features Cancer Res., November 15, 2002; 62(22): 6598 - 6605. [Abstract] [Full Text] [PDF]

				C. Amendt, A. Mann, P. Schirmacher, and M. Blessing Resistance of keratinocytes to TGF{beta}-mediated growth restriction and apoptosis induction accelerates re-epithelialization in skin wounds J. Cell Sci., May 15, 2002; 115(10): 2189 - 2198. [Abstract] [Full Text] [PDF]