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Flow Cytometric Analysis of Androgen Receptor Expression in Human Prostate Tumors and Benign Tissues¹

Division of Experimental Therapeutics [A. K., A. O., W. Y., R. D., D. S.] and Department of Radiation Oncology, Urology Department [B. L. L.], Sylvester Cancer Center, University of Miami School of Medicine, Miami, Florida 33136

Androgen receptor (AR) plays an important role in growth and hormonal therapy of human prostate tumors. Immunohistochemical analysis of AR expression, a nonquantitative technique, is currently used for screening of receptor expression in prostate tissues. The present report describes a laser flow cytometric method for monitoring AR expression in human cell lines and in archival formalin-fixed paraffin-embedded prostate tissues and tumors. Multiparametric flow analysis can be used for simultaneous detection of other cellular markers (e.g., DNA aneuploidy), and by gated analysis, AR expression in subpopulations of a tumor can be quantitatively determined.

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