This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ishikura, H. Articles by Monden, Y. Search for Related Content PubMed PubMed Citation Articles by Ishikura, H. Articles by Monden, Y.

Suppression of Mediastinal Metastasis by Uracil-Tegafur or cis-Diamminedichloroplatinum(II) Using a Lymphogenous Metastatic Model in a Human Lung Cancer Cell Line

Second Department of Surgery, School of Medicine, University of Tokushima, Tokushima 770-8503, Japan

Purpose and Experimental Design: The extent of lymphatic metastasis is the most important factor in the prognosis for non-small cell lung cancer (NSCLC). Therefore, suppression of lymphatic metastasis provides an improvement in survival time in lung cancer patients. We established a new patient-like model for lung cancer metastasis by orthotopic implantation in severe combined immunodeficiency (SCID) mice and demonstrated the lymphogenous spread histologically using human NSCLC cell lines. The cardinal features of this model are a simple procedure and a similarity to the metastatic form of human lung cancer. The purpose of this study is to assess the inhibitory action of uracil-tegafur (UFT) and cis-diamminedichloroplatinum(II) (CDDP) on lymphatic metastasis and life span prolongation in our lymphogenous metastatic model system using SCID mice.

Results: The inhibition ratios of mediastinal lymph node metastasis were 86.2, 94, and 92.1% for 12 mg/kg body UFT, 17 mg/kg body UFT, and 10 mg/kg body CDDP, respectively. The administration of anticancer drugs prolonged the life span by 4.6 days (17 mg/kg body UFT) and 8 days (10 mg/kg body CDDP) in MST.

Conclusion: We demonstrated that UFT alone and CDDP alone suppressed mediastinal metastasis and prolonged the life span in our lymphogenous metastatic model. Regardless of the administration route and characteristics of anticancer drugs, cytostatic or cytotoxic, our model is capable of evaluating the inhibitory effect of drugs on lymphatic metastasis. This model should make an important contribution to our understanding of the mechanism and selection of drugs for antilymphatic metastasis in lung cancer.

This article has been cited by other articles:

				H. Fujino, K. Kondo, H. Ishikura, H. Maki, H. Kinoshita, T. Miyoshi, Y. Takahashi, N. Sawada, H. Takizawa, T. Nagao, et al. Matrix metalloproteinase inhibitor MMI-166 inhibits lymphogenous metastasis in an orthotopically implanted model of lung cancer Mol. Cancer Ther., September 1, 2005; 4(9): 1409 - 1416. [Abstract] [Full Text] [PDF]

				J. Brabek, S. S. Constancio, P. F. Siesser, N.-Y. Shin, A. Pozzi, and S. K. Hanks Crk-Associated Substrate Tyrosine Phosphorylation Sites Are Critical for Invasion and Metastasis of Src-Transformed Cells Mol. Cancer Res., June 1, 2005; 3(6): 307 - 315. [Abstract] [Full Text] [PDF]

				Y. Zou, H. Fu, S. Ghosh, D. Farquhar, and J. Klostergaard Antitumor Activity of Hydrophilic Paclitaxel Copolymer Prodrug Using Locoregional Delivery in Human Orthotopic Non-Small Cell Lung Cancer Xenograft Models Clin. Cancer Res., November 1, 2004; 10(21): 7382 - 7391. [Abstract] [Full Text] [PDF]