This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yonemura, Y. Articles by Sasaki, T. Search for Related Content PubMed PubMed Citation Articles by Yonemura, Y. Articles by Sasaki, T.

Prediction of Peritoneal Micrometastasis by Peritoneal Lavaged Cytology and Reverse Transcriptase-Polymerase Chain Reaction for Matrix Metalloproteinase-7 mRNA

Second Department of Surgery, School of Medicine [Y. Y., T. F., I. N., E. B., T. Saw., K. K.], and Experimental Therapeutics, Cancer Research Institute [Y. E., T. Sas.], Kanazawa University, Kanazawa 920-8640, Japan; Virology Division, National Cancer Center Research Institute, Chuoh-Ku, Tokyo 104, Japan [K. S.]; Department of Surgery, Asan Medical Center, College of Medicine, Ulsam University, Songpa-Ku, Seoul 138-040, Korea [B-S. K.]

Purpose: Peritoneal dissemination is the most common cause of death associated with gastric cancer. In this study, we report the significance of molecular diagnosis of peritoneal dissemination by means of matrix metalloproteinase-7 (MMP-7) reverse transcriptase-PCR (RT-PCR) assay using preoperative peritoneal wash fluid.

Experimental Design: Preoperative peritoneal lavage by paracentesis was performed on 152 patients with gastric cancer. The peritoneal lavaged fluid was subjected to RT-PCR analysis with primers specific for MMP-7 and conventional cytological Papanicolaou examination.

Results: The MMP-7 RT-PCR assay was able to detect cancer cells at densities even lower than 10 cells/sample. There was no signal of MMP-7 mRNA from mesothelial cells, fibroblasts, peripheral blood, and lavaged fluid from patients with benign disease. Cytological examination and MMP-7 RT-PCR assay results were positive for 27 (18%) and 28 (18%) samples, respectively. The sensitivity for the prediction of peritoneal dissemination by cytology and MMP-7 RT-PCR assay were 46% and 33%, but the combination analysis using both parameters improved the sensitivity rate with 62%. Logistic regression analysis revealed that the cytological examination and MMP-7 RT-PCR assay are independent predictors of peritoneal dissemination.

Conclusion: The combination of cytological examination and RT-PCR assay of preoperative peritoneal lavaged fluid is a highly efficient and reliable method for the selection of patients for adjuvant i.p. chemotherapy.

This article has been cited by other articles:

				S. Gretschel, C. Schick, U. Schneider, L. Estevez-Schwarz, A. Bembenek, and P. M. Schlag Prognostic Value of Cytokeratin-Positive Bone Marrow Cells of Gastric Cancer Patients Ann. Surg. Oncol., February 1, 2007; 14(2): 373 - 380. [Abstract] [Full Text] [PDF]

				Y. Kodera, H. Nakanishi, S. Ito, Y. Mochizuki, Y. Yamamura, M. Fujiwara, K. Hibi, K. Ito, S. Akiyama, M. Tatematsu, et al. Detection of Disseminated Cancer Cells in Linitis Plastica-type Gastric Carcinoma Jpn. J. Clin. Oncol., September 1, 2004; 34(9): 525 - 531. [Abstract] [Full Text] [PDF]

				T. Mori, Y. Fujiwara, Y. Sugita, T. Azama, T. Ishii, K. Taniguchi, K. Yamazaki, S. Takiguchi, T. Yasuda, M. Yano, et al. Application of Molecular Diagnosis for Detection of Peritoneal Micrometastasis and Evaluation of Preoperative Chemotherapy in Advanced Gastric Carcinoma Ann. Surg. Oncol., January 1, 2004; 11(1): 14 - 20. [Abstract] [Full Text] [PDF]