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Prognostic Score of Gastric Cancer Determined by cDNA Microarray¹

Department of Surgery, Medical Institute of Bioregulation, Kyushu University, Beppu 874-0838 [H. I., A. M., K. M., M. M.], and Department of Surgery, Oita Prefectural Hospital, Oita 870-0855 [H. U.], Japan

Purpose: To estimate gastric cancer patients’ prognosis more comprehensively,we tried to develop a prognostic scoring system using a cDNA microarray.

Experimental Design: RNA was extracted from tumor/normal paired samples of 43 patients with gastric cancer, and cDNA microarray hybridization was performed.

Results: We selected 78 genes that were differentially expressed between aggressive and nonaggressive groups with respect to five conventional pathological factors. Next, we determined a coefficient for each gene. Thereafter a prognostic score was calculated by summing-up the value for each gene. It ranged from -47 to 201 with a median of 114. There were two peaks in its distribution. Ten of 11 patients who were alive with no evidence of recurrence >5 years after the operation showed a score of <100 points, whereas all 19 patients who died of disease showed >100 points. In 13 patients who were alive but the follow-up time was <5 years, 2 of the 3 patients with >100 points revealed recurrent disease during the follow-up.

Conclusions: These findings demonstrate that such a system with cDNA microarray can contribute to the comprehensive analysis of malignant behavior of the tumor and may provide accurate information on prognosis.

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