| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Molecular Oncology, Markers, Clinical Correlates |
Departments of Pathology [A. A., E. E., Y. N., L. S., P. H. W.] and Biochemistry and Molecular Biology [L. C. M.], University of Manitoba, Faculty of Medicine, Winnipeg, Manitoba, R3E OW3 Canada, and Cancer Research United Kingdom Molecular Oncology Laboratory, University of Oxford, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom [H. S., C. C. W., A. L. H.]
Purpose: The risk of recurrence and progression of ductal carcinoma in situ (DCIS) of the breast is best designated by morphological indicators, including the presence of necrosis. Our purpose was to identify molecular alterations underlying progression of DCIS.
Experimental Design: We have compared gene expression within a cohort of six cases of DCIS with necrosis (DCISnecrosis+) and four cases without necrosis (DCISnecrosis-) using microdissection and cDNA microarray.
Results: A set of 69 cDNAs from a group of 1181 was identified that were consistently differentially expressed. Among this set, the mRNA for angio-associated migratory cell protein and a serine threonine protein kinase, nuclear Dbf2 related, were consistently higher in DCISnecrosis+ and were also found to be overexpressed in the T47D breast cancer cell line subjected to hypoxia. Further study of angio-associated migratory cell protein by quantitative reverse transcriptase-PCR and in situ hybridization analysis of 37 cases of DCIS confirmed higher mRNA expression in DCISnecrosis+ (P = 0.0095).
Conclusions: This study shows that although levels of gene expression are mostly similar between morphologically different DCIS, consistent differences in expression of a subset of genes can be identified between DCIS with and without necrosis.
This article has been cited by other articles:
|
|
 |
|
  F. Vogt, A. Zernecke, M. Beckner, N. Krott, A.-K. Bosserhoff, R. Hoffmann, M. A.M.J. Zandvoort, T. Jahnke, M. Kelm, C. Weber, et al. Blockade of Angio-Associated Migratory Cell Protein Inhibits Smooth Muscle Cell Migration and Neointima Formation in Accelerated Atherosclerosis J. Am. Coll. Cardiol., July 22, 2008; 52(4): 302 - 311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vincent-Salomon, C. Lucchesi, N. Gruel, V. Raynal, G. Pierron, R. Goudefroye, F. Reyal, F. Radvanyi, R. Salmon, J.-P. Thiery, et al. Integrated Genomic and Transcriptomic Analysis of Ductal Carcinoma In situ of the Breast Clin. Cancer Res., April 1, 2008; 14(7): 1956 - 1965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. W. Pearson and T. Hunter Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells J. Cell Biol., December 31, 2007; 179(7): 1555 - 1567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J. Walton, J. Heitman, and A. Idnurm Conserved Elements of the RAM Signaling Pathway Establish Cell Polarity in the Basidiomycete Cryptococcus neoformans in a Divergent Fashion from Other Fungi Mol. Biol. Cell, September 1, 2006; 17(9): 3768 - 3780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Stefansson and D. L. Brautigan Protein Phosphatase 6 Subunit with Conserved Sit4-associated Protein Domain Targets I{kappa}B{epsilon} J. Biol. Chem., August 11, 2006; 281(32): 22624 - 22634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hergovich, S. J. Bichsel, and B. A. Hemmings Human NDR Kinases Are Rapidly Activated by MOB Proteins through Recruitment to the Plasma Membrane and Phosphorylation Mol. Cell. Biol., September 15, 2005; 25(18): 8259 - 8272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang, R. Cui, X. Cheng, and J. Du Antiapoptotic Effect of Serum and Glucocorticoid-Inducible Protein Kinase Is Mediated by Novel Mechanism Activating I{kappa}B Kinase Cancer Res., January 15, 2005; 65(2): 457 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Devroe, H. Erdjument-Bromage, P. Tempst, and P. A. Silver Human Mob Proteins Regulate the NDR1 and NDR2 Serine-Threonine Kinases J. Biol. Chem., June 4, 2004; 279(23): 24444 - 24451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Stegert, R. Tamaskovic, S. J. Bichsel, A. Hergovich, and B. A. Hemmings Regulation of NDR2 Protein Kinase by Multi-site Phosphorylation and the S100B Calcium-binding Protein J. Biol. Chem., May 28, 2004; 279(22): 23806 - 23812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Burstein, K. Polyak, J. S. Wong, S. C. Lester, and C. M. Kaelin Ductal Carcinoma in Situ of the Breast N. Engl. J. Med., April 1, 2004; 350(14): 1430 - 1441. [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Adam, J. Berry, J. A. Loader, K. L. Tyson, G. Craggs, P. Smith, J. De Belin, G. Steers, F. Pezzella, K. F. Sachsenmeir, et al. Arylamine N-Acetyltransferase-1 Is Highly Expressed in Breast Cancers and Conveys Enhanced Growth and Resistance to Etoposide in Vitro Mol. Cancer Res., September 1, 2003; 1(11): 826 - 835. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
