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Altered Subcellular Localization and Low Frequency of Mutations of ING1 in Human Brain Tumors

¹ Departments of Medical Biochemistry and Oncology and Southern Alberta Cancer Research Center, The University of Calgary, Calgary, Alberta, Canada;
² Department of Surgery II, Nagoya City University Medical School, Nagoya, Japan;
³ Division of Epidemiology, Prevention and Screening, Tom Baker Cancer Center, Calgary, Alberta, Canada;
⁴ Genome Prairie, Calgary, Alberta, Canada; and
⁵ Department of Oncology and Clinical Neuroscience, The University of Calgary and Tom Baker Cancer Center, Calgary, Alberta, Canada

Purpose: Clinical and experimental evidence suggest that the p33^ING1b candidate tumor suppressor functionally cooperates with p53 in controlling biochemical and biological functions. Because p53 is frequently mutated in brain tumors and the ING1 locus maps to a site of which the loss is associated with gliomas, we analyzed the mutation and expression profiles of ING1B in human brain tumors. Here we present the first report of ING1 expression and mutation analyses in human brain tumor samples and malignant glioma cell lines.

Experimental Design: Expression and mutation analyses of ING1B together with subcellular localization studies of ING1 proteins were performed on 29 brain tumor specimens and 6 human glioma cell lines.

Results: A single point mutation (3.5%) was detected in the 29 brain tumor specimens analyzed. This missense mutation occurred in a sequence reported previously to confer nuclear translocation properties to p33^ING1b. Interestingly, overexpression and subcellular mislocalization of p33^ING1b were observed in all 29 of the brain tumor specimens and some glioma cell lines. In tumor samples, ING1 proteins aberrantly localized to the cytoplasm, and to a lesser extent, to the nucleus of glioma cells.

Conclusions: Our data indicate that although mutations of ING1 seem to be infrequent in human brain tumors, deregulated expression and mislocalization of ING1 proteins, particularly the p33^ING1b isoform, are common events in gliomas and glioblastomas.

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