| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Molecular Oncology, Markers, Clinical Correlates |
Department of Molecular Oncology [H. T., A. B., M. T., C. K., D. S. B. H.], Division of Biostatistics, John Wayne Cancer Institute [H-J. W.], Department of Pathology, Saint John’s Health Center [R. T.], Santa Monica, California 90404, and Departments of Surgery [S. S.] and Pathology [D. W.], McLaren Regional Medical Center, Michigan State University, Flint, Michigan 48532
Purpose: Both c-MET and vascular endothelial growth factor (VEGF)-C expression are important factors in primary carcinoma progression. We hypothesized that overexpression of c-MET and/or VEGF-C mRNA in primary colorectal cancer (CRC) can predict tumor invasion and regional metastasis.
Experimental Design: The level of c-MET and VEGF-C mRNA expression was assessed using a quantitative RT-RealTime PCR assay on early stage primary CRC tumors (n = 36).
Results: The c-MET mRNA copy number ranged from 1.18 x 102 to 1.11 x 106 copies (median 5.17 x 104) per 250 ng of RNA from CRC specimens. c-MET mRNA copies in CRC specimens was significantly higher than that from normal colon mucosal epithelium (P = 0.0001). c-MET mRNA copies significantly correlated with the depth of invasion: T1 versus T2, P = 0.007; T1 versus T3/T4, P = 0.0001; T1 versus T2 versus T3/T4, P = 0.0005; and T1/T2 versus T3/T4, P = 0.011. c-MET copy number in primary CRC of N1/N2 staged patients was significantly higher than N0 cases (P < 0.03). Expression levels of c-MET mRNA were verified with immunohistochemistry analysis of c-MET protein expression in CRC specimens and normal mucosal epithelium. The VEGF-C mRNA copies of primary CRC assessed ranged from 0 to 1.65 x 105 copies (median 580). Although VEGF-C mRNA copies in CRC primary tumors were significantly higher than normal colon mucosal epithelium (P = 0.0008), it did not correlate with any major clinicopathological parameters of CRC.
Conclusions: This study indicates c-MET mRNA overexpression in primary CRC may be an important prognostic marker for early stage invasion and regional disease metastasis.
This article has been cited by other articles:
|
|
 |
|
  F. Ginty, S. Adak, A. Can, M. Gerdes, M. Larsen, H. Cline, R. Filkins, Z. Pang, Q. Li, and M. C. Montalto The Relative Distribution of Membranous and Cytoplasmic Met Is a Prognostic Indicator in Stage I and II Colon Cancer Clin. Cancer Res., June 15, 2008; 14(12): 3814 - 3822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Singh, S. Subramanian, J. M. Rhodes, and B. J. Campbell Peanut lectin stimulates proliferation of colon cancer cells by interaction with glycosylated CD44v6 isoforms and consequential activation of c-Met and MAPK: functional implications for disease-associated glycosylation changes Glycobiology, July 1, 2006; 16(7): 594 - 601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Patane, S. Avnet, N. Coltella, B. Costa, S. Sponza, M. Olivero, E. Vigna, L. Naldini, N. Baldini, R. Ferracini, et al. MET Overexpression Turns Human Primary Osteoblasts into Osteosarcomas. Cancer Res., May 1, 2006; 66(9): 4750 - 4757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Cao, M. A. Bjorndahl, M. I. Gallego, S. Chen, P. Religa, A. J. Hansen, and Y. Cao Hepatocyte growth factor is a lymphangiogenic factor with an indirect mechanism of action Blood, May 1, 2006; 107(9): 3531 - 3536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takeuchi, J. Kim, A. Fujimoto, N. Umetani, T. Mori, A. Bilchik, R. Turner, A. Tran, C. Kuo, and D. S.B. Hoon X-Linked Inhibitor of Apoptosis Protein Expression Level in Colorectal Cancer Is Regulated by Hepatocyte Growth Factor/C-Met Pathway via Akt Signaling Clin. Cancer Res., November 1, 2005; 11(21): 7621 - 7628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kim, H. Takeuchi, S. T. Lam, R. R. Turner, H.-J. Wang, C. Kuo, L. Foshag, A. J. Bilchik, and D. S.B. Hoon Chemokine Receptor CXCR4 Expression in Colorectal Cancer Patients Increases the Risk for Recurrence and for Poor Survival J. Clin. Oncol., April 20, 2005; 23(12): 2744 - 2753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takeuchi, D. L. Morton, C. Kuo, R. R. Turner, D. Elashoff, R. Elashoff, B. Taback, A. Fujimoto, and D. S.B. Hoon Prognostic Significance of Molecular Upstaging of Paraffin-Embedded Sentinel Lymph Nodes in Melanoma Patients J. Clin. Oncol., July 1, 2004; 22(13): 2671 - 2680. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Resnick, J. Routhier, T. Konkin, E. Sabo, and V. E. Pricolo Epidermal Growth Factor Receptor, c-MET, {beta}-Catenin, and p53 Expression as Prognostic Indicators in Stage II Colon Cancer: A Tissue Microarray Study Clin. Cancer Res., May 1, 2004; 10(9): 3069 - 3075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lastraioli, L. Guasti, O. Crociani, S. Polvani, G. Hofmann, H. Witchel, L. Bencini, M. Calistri, L. Messerini, M. Scatizzi, et al. herg1 Gene and HERG1 Protein Are Overexpressed in Colorectal Cancers and Regulate Cell Invasion of Tumor Cells Cancer Res., January 15, 2004; 64(2): 606 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zou, S. Lim, K. Lee, X. Deng, and E. Friedman Serine/Threonine Kinase Mirk/Dyrk1B Is an Inhibitor of Epithelial Cell Migration and Is Negatively Regulated by the Met Adaptor Ran-binding Protein M J. Biol. Chem., December 5, 2003; 278(49): 49573 - 49581. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
