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Genetic and Epigenetic Changes in p21 and p21B Do Not Correlate with Resistance to Doxorubicin or Mitomycin and 5-Fluorouracil in Locally Advanced Breast Cancer

¹ Department of Medicine, Section of Oncology, Haukeland University Hospital, Bergen; and ² Department of Molecular Biology, University of Bergen, Bergen, Norway

	ABSTRACT

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Purpose: The cyclin-dependent kinase inhibitor p21 acts as a main executor of p53-induced growth arrest. Recently, a second transcript, p21B, was found to code for a protein expressing proapoptotic activity. We investigated p21 and p21B for mutations and epigenetic silencing in locally advanced breast cancers treated with doxorubicin or 5-fluorouracil/mitomycin and correlated our findings with treatment response and TP53 status.

Experimental Design: We used reverse transcription-PCR to analyze p21/p21B mutation status in 73 breast cancer samples. The p21 promoter region was sequenced and analyzed for hypermethylations by methylation-specific PCR. In addition, a selection of patients were analyzed for mutations in the p21B promoter.

Results: The p21 gene was neither mutated nor silenced by promoter hypermethylation in any of the tumors examined. One patient harbored a novel p21 splice variant in addition to the wild-type transcript. We observed two base substitutions in the p21 transcript, C93A and G251A, each affecting six patients (8.2%). The G251A variant had not been reported previously. In 12 patients (16.4%), we observed a novel base substitution, T35C, in p21B. All three base substitutions were observed in lymphocyte DNA and therefore considered polymorphisms. The polymorphisms did not correlate with p21 staining index, treatment response to doxorubicin or 5-fluorouracil/mitomycin, or TP53 status.

Conclusions: Our findings do not suggest that genetic or epigenetic disturbances in p21 or p21B cause resistance to doxorubicin or mitomycin/5-fluorouracil in breast cancer. Future studies should assess potential associations between these novel polymorphisms and breast cancer risk.

	INTRODUCTION

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Although the key factors and functional pathways involved in growth control and apoptosis have been characterized in experimental systems, the interplay between the different factors in response to cytotoxic treatment in human cancers is poorly understood. The tumor suppressor TP53 plays a pivotal role with respect to apoptosis as well as growth arrest and DNA repair (1, 2, 3) . Thus, experimental studies have revealed a key role for p53 (the protein coded for by the TP53 gene) in executing responses to different cytotoxic agents in vitro as well as in animal models (4, 5, 6, 7) . Importantly, we (8) and others (9) have shown that mutations in TP53 are associated with resistance to anthracyclines, as well as to mitomycin in combination with 5-fluorouracil (10) , in breast cancer.

Although TP53 mutations have been found associated with drug resistance, some observations remain to be explained. Our previous studies revealed a strong association between TP53 mutations affecting the L2 or L3 loops of the protein and resistance to doxorubicin or mitomycin/5-fluorouracil in primary breast cancer, but some patients expressed chemoresistance despite harboring wild-type (wt) TP53 (10 , 11) . This led us to postulate that some of these tumors may suffer damage to other genes involved elsewhere in the "p53 pathway" (12) . Recently, we found that a high tumor growth rate, assessed by high mitotic index and Ki67, was associated with a lower response rate in patients harboring wt TP53 but not those harboring a mutated TP53 gene (13) , suggesting that disturbances in the cell cycle could be an important factor in these patients.

The role of p53 regarding the effects of chemotherapy has been related to its apoptotic function. However, the interplay between p53-induced apoptosis and growth arrest is far from clear (14) . The cyclin-dependent kinase inhibitor p21 (the 21-kDa protein encoded by the p21 gene) is thought to be the main downstream executor of growth arrest triggered by p53 activation (15, 16, 17, 18) . p21 may bind cdks-2 or -4, generating tertiary complexes with cyclin D or cyclin E (18, 19, 20, 21, 22, 23, 24, 25, 26, 27) , which interact with several events in cell cycle control.

This study is part of our program evaluating disturbances in different genes involved in the TP53 pathway, using the same patient materials in which we previously characterized TP53 alterations in relation to resistance to doxorubicin (11) and mitomycin therapy (10) . Recently, a novel transcript from the p21 gene, designated p21B, was reported (28) . This transcript partially overlaps the normal p21 transcript but has a p53 promoter binding site independent from the p21 promoter region (Fig. 1) . It translates into a protein with no sequence overlap with p21. Contrary to the p21 protein, p21B has been proposed to regulate apoptosis rather than cell-cycle arrest (28) . To our knowledge, p21B mutation status as well as promoter methylation of p21 (p21B contains no CpG islands in the promoter region) has not been evaluated in breast tumors previously.

View larger version (10K): [in this window] [in a new window]   Fig. 1. The p21 gene. The p21 gene encodes two proteins, p21 and p21B, with no coding sequence overlap, arising from partially overlapping RNA transcripts. The different transcripts are controlled by distinct promoter regions, both with p53-binding elements.

	MATERIALS AND METHODS

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Patients.
This study included patients from two prospective studies addressing the potential role of mutations in TP53 and other genes regarding resistance to treatment with doxorubicin (11) or mitomycin and 5-fluorouracil (10) in locally advanced breast cancers. Both studies were approved by the Regional Ethical Committee. Because the studies were designed to explore causes of chemoresistance, we focused on comparing tumors that showed primary drug resistance (progressive disease within 12 weeks) with the combined group of those showing stable disease or an objective response (8 , 12) . Thus, for the patients treated with doxorubicin, we analyzed all tumors that were resistant to therapy together with a randomly selected subgroup of responding tumors (Table 1 ; Ref. 11 ). Regarding the group of patients who were treated with mitomycin and 5-fluorouracil, we lacked sufficient material for analysis of one of the tumors previously reported to progress on therapy, but analyzed the remaining 33 tumors (Table 1 ; Ref. 10 ). In addition, four patients not participating in these studies were analyzed (Table 1) .

Lymphocyte Cultivation and DNA Purification.
Genomic DNA extracted from lymphocytes was available for six patients expressing one or more polymorphisms in p21/p21B. In addition, we selected lymphocyte DNA from four patients expressing wt p21 and p21B as controls. Lymphocytes were prepared by cultivating 0.4 ml of blood at 37°C for 3 days in 10 ml of Medium 199 (Life Technologies, Inc.) containing 18% FCS (Life Technologies, Inc.). Genomic DNA was purified from lymphocytes and tumor tissue by use of the QIAamp DNA Mini Kit (Qiagen), according to the manufacturer’s instructions, followed by sequencing.

PCR Amplification of p21 and p21B.
A 607-bp cDNA fragment encompassing the complete p21 open reading frame was amplified by nested PCR with the primers p21_n2S and p21_n2AS, followed by primers p21_n1S and p21_n1AS (Table 2) . Observed mutations were verified by PCR amplification of p21 exon 2 from genomic DNA, using primers p21_exon2S and p21_exon2AS (Table 2) . p21B samples were amplified as a 492-bp fragment with use of primers p21B_S1 and p21B_AS1 (Table 2) . The p21B coding sequence is located in p21B exon 2. Because our primers covered a segment of this exon, p21B genomic verification of genetic alterations was done with the same primers and PCR program as the cDNA analysis.

PCR was carried out with Dynazyme EXT DNA poly-merase (Dynazyme) in a 50-µl solution containing 1x PCR buffer, 1.5 mM MgCl₂, 0.5 mM each deoxynucleotide triphosphate, 5% DMSO, 0.2 µM each primer, and 0.5 µl of cDNA or 1 µl of genomic DNA. The p21 PCR conditions were identical for each nested PCR: an initial 5-min denaturation at 94°C; 30 cycles of 30 s at 94°C, 30 s at 47°C, and 1 min at 72°C; and a final 7-min extension at 72°C. PCR on genomic DNA was done with an initial 5-min denaturation at 94°C; 30 cycles of 30 s at 94°C, 30 s at 50°C, and 1 min at 72°C; and a final 7-min extension at 72°C. p21B PCR was done with an initial 5-min denaturation at 94°C; 35 cycles of 30 s at 94°C, 30 s at 55°C, and 1 min at 72°C; and a final 7-min extension at 72°C. Finally, the p21B promoter PCR was done with an initial 5-min denaturation at 94°C; 35 cycles of 30 s at 94°C, 30 s at 55°C, and 90 s at 72°C; and a final 7-min extension at 72°C. After amplification, 15 µl of the PCR products were visualized on a 1% agarose gel.

Cloning of PCR Products.
The sample from one patient gave two distinct bands after the p21 cDNA PCR. Each of the PCR products was cloned into the pCR 2.1-TOPO vector (Invitrogen) for separate analysis. A selection of plasmids was purified by Qiaprep spin mini-prep kit (Qiagen), and the inserts were amplified by M13 PCR (according to instructions provided by Invitrogen) to confirm the insert size.

DNA Sequencing.
Sequencing was done directly on 1 µl of the PCR products and plasmids, using Big Dye terminator mixture (Applied Biosystems), with primer sets p21_n1S and p21_n1AS for p21, p21B_S1 and p21B_AS1 for p21B, and p21B_proS, p21B_proAS, p21_exon1S, and p21B_intron1AS for the p21B promoter segments (Table 2) . After an initial 5-min denaturation at 94°C, the sequencing reaction was carried out for 40 cycles of 10 s at 94°C, followed by 5 s at 55°C and 4 min at 60°C. Twenty plasmids were sequenced to ensure that a possible mutation would be discovered. Capillary gel electrophoresis, data collection, and sequence analysis were performed on an automated DNA sequencer (ABI 3700).

Promoter Methylation Analysis.
p21 promoter methylation status was analyzed by methylation-specific PCR. We modified genomic DNA from patients, using the CpGenome DNA Modification Kit (Intergen), and designed primers specific for methylated (p21_MS1 and p21_MAS1; Table 2 ) and unmethylated (p21_US1 and p21_UAS1; Table 2 ) DNA, each PCR product spanning positions –74 through –207 of the p21 promoter sequence. Methylation-specific PCR was done with AmpliTaq Gold DNA Polymerase (Applied Biosystems) in 50 µl of solution containing 1x PCR buffer, 1.5 mM MgCl₂, 0.5 mM each deoxynucleotide triphosphate, 0.2 µM each primer, and 2 µl of modified genomic DNA. The methylated-specific PCR was carried out for 35 cycles of 30 s at 94°C, 30 s at 61°C, and 1 min at 72°C. The unmethylated-specific PCRs were carried out for 35 cycles of 30 s at 94°C, 30 s at 58°C, and 1 min at 72°C. Both PCRs were done with an initial 5 min of denaturation at 94°C and concluded with 7 min at 72°C. After amplification, 18 µl of the PCR products were visualized on a 3% agarose gel.

To complete the methylation analysis of p21, we also examined the STAT-promoter binding site at position –692 to the p21 transcription start site, designated SIE-1 (29) . This motif has a single CpG island, analyzed previously by restriction enzyme analysis by Chen et al. (29) . In 20 samples from randomly selected patients, we amplified a fragment spanning this sequence after DNA modification (as above), using primers p21_STAT_MS and p21_STAT_MAS (Table 2) . After an initial denaturation for 5 min at 94°C, the PCR was carried out for 35 cycles of 30 s at 94°C, 30 s at 55°C, and 1 min at 72°C, with a final 7-min extension at 72°C. The PCR products were sequenced directly or cloned into the pCR 2.1-TOPO vector (Invitrogen) and sequenced.

Immunohistochemistry.
We previously reported p21 immunostaining for a subgroup of patients belonging to the doxorubicin series as outlined elsewhere (30) . In the present study, we compared immunostaining intensity in the subgroup of tumors for which this parameter was available (n = 35 of the patients treated with doxorubicin) with promoter hypermethylation, gene mutations, or polymorphism status.

	RESULTS

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No mutations in the p21 and p21B transcripts were detected. One tumor expressed an alternate splice variant in addition to the p21 wt transcript. The p21 variant contained a deletion of bp 47–251 of the open reading frame. The p21 transcript, which was observed consistently in repeated PCRs, translated correctly the first 16 amino acid residues, followed by a frameshift and further translation of an additional 61 amino acid residues before a stop codon at the end of exon 2. Noteworthy, the tumor harboring this transcript occurred in a male patient and harbored a silent TP53 polymorphism in position 213 (one of the "nonevaluable" patients; Table 1 )

Samples from 12 of 73 patients expressed p21 polymorphisms (Table 3) . The previously reported C93A (Ser31Arg) polymorphism (31, 32, 33, 34, 35) was found in six patients (8.2%). In addition, we found a single-base substitution, G251A (Arg84Gln), in six patients (8.2%). To our knowledge, this substitution has not been reported previously. No patients harbored both polymorphisms.

Considering the p21B transcript, 12 of 73 patients (16.4%) were found to harbor the same single-base substitution, T35C (Leu12Pro; Table 3 ). Lymphocyte genomic DNA was analyzed for p21B alterations in the same 10 patients as for p21 (above); 3 of these patients harbored concomitant p21B base substitutions in their tumors. The T35C single-base substitution was detected in lymphocyte DNA from all three patients, confirming that the alteration is a polymorphism.

None of the tumors were found to be hypermethylated at the p21 promoter (data not shown). The p21B promoter does not contain CpG islands in relation to the p53 binding site and may not be silenced by methylation.

Eleven tumors did not provide a p21B reverse transcription-PCR product and were therefore subjected to genomic DNA analysis. Because the p21B promoter does not contain CpG islands, this finding could not be caused by promoter hypermethylation. DNA sequencing confirmed wt gene sequences in the coding domain, introns, and the promoter region. Furthermore, lack of a p21B reverse transcription-PCR product was not related to occurrence of the p21 or p21B polymorphisms, nor did it correlate to TP53 mutation status. Notably, each of the tumors lacking a p21B reverse transcription-PCR product expressed normal levels of the p21 transcript. For those patients harboring a p21 or a p21B base substitution, genomic sequencing revealed equal amounts of the wt and the variant allele; thus, lack of p21B expression is not due to large gene deletions, mutations, or loss of heterozygosity.

Interestingly, although we observed no correlation between the p21^G251A and p21B^T35C polymorphisms, we did find a nonsignificant correlation between expression of the p21^C93A and p21B^T35C polymorphisms (P = 0.0521, Fisher exact test; Table 3 ). Neither the two p21 polymorphisms nor the p21B^T35C polymorphism correlated with TP53 mutation status or to treatment response.

	DISCUSSION

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The cyclin kinase inhibitor p21 is thought to be a key executor of p53-induced growth arrest. In addition, it is known to be transcriptionally regulated by factors such as c-Myc and Miz-1 (36 , 37) , transforming growth factor-ß (38) , IFN-, and STAT1 (39) , and it may be regulated at the protein level through direct binding to other proteins, including SET (40) and phosphorylation by Akt (41) . Although p21 may bind cdk4 (cyclin D) as well as cdk2 (cyclin E), the evidence at present suggests that it stabilizes the cdk4–cyclin D complex but inhibits the cdk2–cyclin E complex (42 , 43) , subsequently inhibiting phosphorylation and activation of the retinoblastoma protein in the S-phase (27) . Loss of p21 function may lead to activation of the cdk2–cyclin E complex, and recent evidence has linked overexpression of this cyclin, in particular the low molecular forms, to a high risk of relapse in breast cancer (42 , 43) .

The aim of this study was to explore p21 status in relation to chemoresistance. Although in previous studies our group (10 , 11) found that certain mutations in the TP53 gene are associated with resistance to doxorubicin and mitomycin treatment in breast cancer, we observed tumors showing chemoresistance despite harboring wt TP53 (44) . In the present study, we explored whether disturbances in the p21 gene could explain some of these findings. Our previous finding (30) that p21 immunostaining did not add predictive information does not exclude a potential role for genetic or epigenetic events in a minor fraction of patients. Although rare (32 , 33 , 45, 46, 47, 48, 49, 50, 51, 52, 53) , mutations in the p21 gene could be of critical importance in some tumors. Furthermore, because a lack of protein staining could be due to several mechanisms, previous findings do not distinguish certain epigenetic events, such as permanent gene silencing through promoter hypermethylation, from other "physiological" mechanisms.

The finding of a truncated p21 transcript in a tumor harboring wt p21 is interesting. This patient was not evaluable for response to chemotherapy; thus, we do not know whether this transcript could influence drug sensitivity. To evaluate potential biological functions of this transcript could be an issue for future studies.

We observed two polymorphisms in the p21 transcript, each occurring in 8.2% of the patients, but no somatic mutations or promoter methylation. The Ser31Arg polymorphism has been linked to cancer susceptibility in one study (54) ; however, other investigators could not confirm this finding (34 , 55, 56, 57) . Although the G251A (Arg84Gln) base substitution, to our knowledge, has not been reported previously, the fact that it was found in normal tissue from patients harboring the alteration in their tumor strongly suggests that this is a polymorphism.

Recently, a new p53 promoter binding site in the p21 gene was reported (28) . Through this site, p53 induces transcription of a novel p21 variant, named p21B, with a coding sequence with no overlap to p21 (Fig. 1) . A recent in vitro study suggested a role for p21B with respect to apoptosis (28) . We observed an apparent p21B polymorphism, Leu12Pro, in 12 of 73 (16.4%) patients, but no somatic mutations. Although reverse transcription-PCR did not reveal a p21B mRNA transcript in some of our patients, DNA sequencing and evaluation of promoter integrity excluded p21B-specific mutations in these patients, suggesting that this finding could be due to method problems.

Importantly, none of the p21 and p21B polymorphisms were associated with response to therapy, TP53 status, or p21 immunostaining.

In conclusion, no mutations or promoter hypermethylation in p21 or p21B were identified in any of 73 locally advanced breast cancers. Although we identified two polymorphisms affecting p21 and one polymorphism affecting p21B, none of these polymorphisms correlated with treatment outcome or TP53 status. The fact that two of these polymorphisms, despite being found in 8.2% (G251A) and 16.4% (T35C) of our patients, have not been reported previously raises the question of whether these polymorphisms may be seen among breast cancer patients in particular and thus are associated with disease risk. This hypothesis should be explored in future studies.

	ACKNOWLEDGMENTS

 
We are grateful to Professor Mitch Dowsett, Royal Marsden Hospital, London, for the p21 immunostaining results and to Dagfinn Ekse and Hildegunn Helle for technical assistance.

	FOOTNOTES

 
Grant support: Norwegian Cancer Society and the Haukeland Hospital Program for Gene Therapy. V. Staalesen is a recipient of a fellowship from the Norwegian Cancer Society.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Requests for reprints: Per E. Lønning, Department of Medicine, Section of Oncology, Haukeland University Hospital, N-5021 Bergen, Norway. Phone: 47-55-972027; Fax: 47-55-972046; E-mail: per.lonning{at}helse-bergen.no

Received 12/23/03; revised 2/16/04; accepted 2/18/04.

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