Clinical Cancer Research 11, , , . doi:
© 2009 American Association for Cancer Research
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Fig. 6. Effect of BMS-354825 on cell invasion. Cells were plated onto Matrigel-coated filters in a modified Boyden chamber. Cells were allowed to attach and spread for 16 hours before the addition of 10 nmol/L (for HNSCC cells) or 50 nmol/L (for NSCLC cells) BMS-354825 to the upper chamber and conditioned medium to the lower chamber. After 24 hours, cells still in the upper chamber were removed and cells that had invaded through the Matrigel were stained and counted. In parallel, cells treated identically were assessed for viability and cell number with trypan blue. The average number of cells per field is expressed as a percentage of the control after normalizing for cell number. The concentration of BMS-354825 was lowered in this experiment to minimize its effect on the absolute cell number. In all cases, when treated samples are compared with corresponding controls, P < 0.05.
