The conventional therapies like radiation, chemotherapy, and surgery used for treatment of head and neck cancers have served well, but they are not without significant side effects. The development of agents that are non-toxic to normal cells, but delay onset and/or progression of human head-and-neck/oral squamous-cell carcinomas (HNOSCC) may lead to more effective treatment of the disease and a reduction of morbidity and mortality. Prevention of cancer through dietary intervention recently has received an increasing interest, and dietary derivatives have become not only important potential chemopreventive, but also therapeutic, natural agents. Use of these chemopreventive agents is the most practical approach to reduce the morbidity and mortality and to delay the process of carcinogenesis.
The present study is focused to determine potential of Compound X (derived from Ayurvedic medicinal plant) for treatment of HNOSCC. Oral cancer cells (SCC4) showed reduced cell viability on treatment with Compound X in dose and time dependent manner. FACS analysis showed Compound X induces apoptosis in SCC4 cells. To determine the novel molecular targets of compound X, whole cells lysates of SCC4 cells (treated / untreated control) were separated using 2-dimensional gel electrophoresis. Differentially expressed spots were cut, eluted, trypsinized and identified by MALDI-TOF MS/MS. Pathway analysis showed these proteins to be an integral part of signaling cascades involved in cellular inflammation, proliferation, apoptosis, and differentiation, Thus our results suggests that Compound X may serve as a potential therapeutic agent for HNOSCC.
- American Association for Cancer Research