Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
    • CME
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • CCR Focus Archive
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citation
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Clinical Cancer Research
Clinical Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
    • CME
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • CCR Focus Archive
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citation
    • Author/Keyword
  • News
    • Cancer Discovery News
Imaging, Diagnosis, Prognosis

Pyrophosphorolysis-activated polymerization detects circulating tumor DNA in metastatic uveal melanoma.

Jordan Madic, Sophie Piperno-Neumann, Vincent Servois, Aurore Rampanou, Maud Milder, Benedicte Trouiller, David Gentien, Stéphanie Saada, Franck Assayag, Aurélie Thuleau, Fariba Némati, Didier Decaudin, Francois-Clement Bidard, Laurence Desjardins, Pascale Mariani, olivier lantz and Marc-Henri Stern
Jordan Madic
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sophie Piperno-Neumann
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vincent Servois
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aurore Rampanou
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maud Milder
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benedicte Trouiller
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David Gentien
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stéphanie Saada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Franck Assayag
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aurélie Thuleau
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fariba Némati
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Didier Decaudin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Francois-Clement Bidard
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laurence Desjardins
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pascale Mariani
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
olivier lantz
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marc-Henri Stern
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/1078-0432.CCR-12-0309
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Purpose: To develop a molecular tool to detect circulating tumor-derived DNA (ctDNA) in the plasma from patients with uveal melanoma (UM) as a marker of tumor burden and monitor treatment efficacy. Experimental Design: A real-time PCR was developed based on bi-directional pyrophosphorolysis-activated polymerization (bi-PAP) for the quantification of ctDNA using 3'blocked primer pairs specific for the 3 recurrent mutually exclusive mutations of Gα subunits GNAQ and GNA11. Results: Sensitivity and specificity of bi-PAP were assessed on serial dilutions of tumor DNA in normal DNA for the 3 recurrent mutations. Each assay could detect a single mutated molecule per reaction, while 104 copies of normal DNA were not detected. CtDNA was readily detected in plasma of mice bearing UM xenografts in amounts proportional to circulating human DNA. Finally, plasma were almost always found positive (20 out of 21 tested patients) in a prospective analysis of metastatic UM patients. Conclusions: Bi-PAP assays detect and quantify ctDNA in metastatic UM patients. A prospective study is ongoing to assess the clinical usefulness of ctDNA level in UM.

  • Received January 29, 2012.
  • Revision received April 19, 2012.
  • Accepted May 9, 2012.
  • Copyright © 2012, American Association for Cancer Research.
Next
Back to top

Published OnlineFirst May 29, 2012
doi: 10.1158/1078-0432.CCR-12-0309

Open full page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Clinical Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Pyrophosphorolysis-activated polymerization detects circulating tumor DNA in metastatic uveal melanoma.
(Your Name) has forwarded a page to you from Clinical Cancer Research
(Your Name) thought you would be interested in this article in Clinical Cancer Research.
Citation Tools
Pyrophosphorolysis-activated polymerization detects circulating tumor DNA in metastatic uveal melanoma.
Jordan Madic, Sophie Piperno-Neumann, Vincent Servois, Aurore Rampanou, Maud Milder, Benedicte Trouiller, David Gentien, Stéphanie Saada, Franck Assayag, Aurélie Thuleau, Fariba Némati, Didier Decaudin, Francois-Clement Bidard, Laurence Desjardins, Pascale Mariani, olivier lantz and Marc-Henri Stern
Clin Cancer Res May 29 2012 DOI: 10.1158/1078-0432.CCR-12-0309

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Pyrophosphorolysis-activated polymerization detects circulating tumor DNA in metastatic uveal melanoma.
Jordan Madic, Sophie Piperno-Neumann, Vincent Servois, Aurore Rampanou, Maud Milder, Benedicte Trouiller, David Gentien, Stéphanie Saada, Franck Assayag, Aurélie Thuleau, Fariba Némati, Didier Decaudin, Francois-Clement Bidard, Laurence Desjardins, Pascale Mariani, olivier lantz and Marc-Henri Stern
Clin Cancer Res May 29 2012 DOI: 10.1158/1078-0432.CCR-12-0309
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Advertisement

Related Articles

Cited By...

More in this TOC Section

  • TP53 in Breast Cancer
  • FDOPA PET Survival Predictions for Glioma
  • In vivo Fluorescence Lifetime Imaging for Monitoring the Cancer Treatment
Show more Imaging, Diagnosis, Prognosis
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • CCR Focus Archive
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Clinical Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Clinical Cancer Research
eISSN: 1557-3265
ISSN: 1078-0432

Advertisement