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Subcellular localization of KLF4 in vitro. HEK293 cells were analyzed 48 hours after transfection of KLF4 (shown in red) and green fluorescent protein (GFP, shown in green). Nuclear localization was determined by comparison to DAPI-stained nuclei (shown in blue). Many cells exhibit a strict nuclear localization of KLF4, indicated by a pink color in GFP-negative cells. In addition, some cells exhibit a perinuclear rim of KLF4, resulting in yellow and green zones in the cytoplasm of GFP-positive cells.