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Double staining of 293T cells transfected with IRTA2 or CD30 plasmids. Cells were transfected separately with either plasmid, detached, combined in a 1:1 ratio, and seeded again before staining. The green color is associated with IRTA2 expressed on the plasma membrane probed by anti-IRTA2 MAb F56. The red color on different cells is associated with CD30. Nuclei are stained blue. For further details see Ise et al. on page 87.