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Accumulation of a fluorescein-labeled cisplatin (green) in the lysosomes (red) of the human ovarian carcinoma cell line 2008 is depicted as yellow. Cells were incubated simultaneously with fluorescein-labeled cisplatin and the lysosomal marker Lysotracker red before being fixed and stained with the nuclear dye Hoechst 33324. Microscopy was performed at UCSD Cancer Center Digital Imaging Shared Resource using a DeltaVision deconvoluting microscope system (Applied Precision, Inc., Issaquah, WA). For further details, please see Safei et al. in this issue. Double staining of 293T cells transfected with IRTA2 or CD30 plasmids. Cells were transfected separately with either plasmid, detached, combined in a 1:1 ratio, and seeded again before staining. The green color is associated with IRTA2 expressed on the plasma membrane probed by anti-IRTA2 MAb F56. The red color on different cells is associated with CD30. Nuclei are stained blue. For further details see Ise et al. on page 87.