Table 1.

Antibody source, pretreatment, dilution, and incubation duration for tumor markers

Primary antibodyCloneSpeciesManufacturerCatalog no.PretreatmentDilutionDiluentIncubation durationSecondary antibodyDetection method
FOXP-1RabbitSigma AldrichHPA003876-100ULVS6a,b1:50BSAOVNMACH-2 Rabbit
SKP22C8D9MouseInvitrogen18-7334VSEa,c1:50BSA90 minMACH-2 Mouse
CD44IM7MouseEbioscience14-0441VS6a,b1:200BSA45 minENV Mouse
P531801MouseOncogeneOP09-100ugVS61:200BSA45 minMACH-2 Mouse
CD10SS2/36MouseDakoM0727PCEc,d1:100BSA45 minENV Mouse
BCL2124MouseDakoM0887VSEa,c1:300BSA45 minENV Mouse
Ki-67MIB1MouseDakoM7240VS6a,b1:100CaCl45 minMACH-2 Mouse
CD30BERH2MouseDakoM0751VS6a,b1:40BSA45 minENV Mouse
CD43DF-T1MouseDakoM0786VS6a,b1:100BSA45 minENV Mouse
CD21IF8MouseDakoM0784PRO Ke1:20BSA45 minENV Mouse
Cyclin D2DCS-3.1 + DCS-5.2RabbitAbcamab3087VSEa,c1:200BSA2 hENV Rabbit
Cyclin ERabbitAbcamab52189VSEa,c1:50BSAOVNENV Rabbit
cMYCY69RabbitEpitomics1472-1VSEa,c for 40 min1:50BSAOVNENV Rabbit
p27/Kip1MouseBD Transduction Laboratory610241VS6a,b1:200BSA2 hENV Mouse
SurvivinRabbitNovus BiologicalNB500-201VS6a,b1:50BSA45 minENV Rabbit
BAXB9MouseSanta Cruzsc-7480PC10d,f1:20BSAOVNENV Mouse
Galectin 3M3/38RatBiolegend125402VS6a,b1:50BSAOVNRb anti ratENV Rabbit
BLIMP16D3RatSanta Cruzsc-47732VSEa,c1:50BSA45 minRb anti ratENV Rabbit
BCL6PG-B6PMouseDakoM7211VSEa,c1:30BSA45 minENV Mouse
PKC-beta 2Y125RabbitAbcamab32026VSEa,b1:200BSAOVNENV Rabbit
MUM1MUM1pMouseDakoM7259PCEc,d1:100BSA45 minENV Mouse
LMO2SP51RabbitSpring BioscienceM3510VSEa,c1:500BSA45 minNovolink
MMP9RabbitDakoA0150PC10d,f1:100BSA60 minCSA Rabbit linkCSA

Abbreviations: BSA, bovine serum albumin; OVN, overnight.

  • aVegetable steamer, 95°C for 25 minutes.

  • b0.01 mol/L sodium citrate buffer, pH = 6.00.

  • c0.001 mol/L EDTA, pH = 8.0.

  • dPressure cooker, pressure cooker at 115°C for 2 to 3minutes.

  • eProteinase K, at 37°C for 10 minutes.

  • f0.05 mol/L Tris, pH = 10.00.