Table 1.

Apoptosis data on a panel of cell lines

Cell lineCell typeCXCR4 expressionbAdjusted percent apoptosis
RamosaLymphoma++++71
NamalwaaLymphoma++++30
RajiaLymphoma++++15
DHL6aLymphoma+3
L540aLymphoma+++35
HL-60AML++31
Nomo-1AML++++34
KG-1AML++8
MOLP-8MM++19
RPMI-8226MM++17
MM.1SMM+15
U226MM+22
JJN-3RMM++31
OPM2MM++17
L-363MM+16
MV-4-11MM++1
MJTCL++9
HHTCL+++9
HuT78TCL+22
CCRF-CEMaALL+++45
NKLNK+++36
KHYG-1NK+10
NK-92NK++48
Human primaryaB (CD19+)++17
Human primaryaT (CD3+)+6
Human primary*Monocytes (CD14+)++24

NOTE: Cells were incubated with 10 to 330 nmol/L MDX-1338 or isotype control at 37°C for 24 hours. For a subset of cells, a cross-linking antibody (goat anti-human IgG Fc–specific polyclonal Ab) was added at 6-fold excess. Cells were then resuspended in Annexin V binding buffer and stained with Annexin V-APC and 7-AAD or PI. Cells were then washed, resuspended in Annexin V binding buffer, and analyzed with a FACSArray system and FlowJo software.

Abbreviations: MM, multiple myeloma; TCL, T-cell lymphoma.

  • aWithout cross-linker.

  • bCXCR4 expression key: MFI with 10 nmol/L Ab (Score; +); 400–2000 (+); 2,000–10,000 (++);10,000–50,000 (+++); 50,000–250,000 (++++).