Table 1.

Summary of platforms and assays to study T-cell modulation

PlatformAssays
High-throughput arrays
  • Transcriptional profiling

  • Proteomic profiling

  • Cytokine profiling

  • Immune subset identification

 
Flow cytometry
  • Surface and intracellular marker detection

  • Functional assays (cytolysis, degranulation, cytokine production)

 
Deep sequencing
  • Detection of specific TCR clonotypes

 
Biochemical
  • Multiplex detection of soluble factors (Luminex, mesoscale, bead array)

  • ELISA