Table 3.

Ct values and calculated EBV copies for the calibrators and the plasma samples when two different PCR master mixes were used

CalibratorPrepared Master Mix from ABI componentsRoche Master Mix
Expected valueObserved read 1Observed read 2Observed read 1Observed read 2
Copies/5 μLCopies/5 μLCtCopies/5 μLCtCopies/5 μLCtCopies/5 μLCt
1500060,45623.7759,45423.7913,15323.2813,00123.30
600036,28324.4533,59424.565,64524.645,36224.72
6004,74427.164,30627.2955128.3759028.26
12093329.3388329.4013030.7011230.93
6031130.7938230.527531.576331.86
3015831.6918131.512433.383232.93
158232.557832.631334.421034.77
7.52634.091434.92835.131034.83
3.751734.65935.57436.23635.69
Copies/mLCtCopies/mLCt
Plasma 141,83128.55463830.12
Plasma 237,34928.70443230.19
Plasma 343,81228.49490230.03
Plasma 450,68028.30484930.05

NOTE: Expected and observed values are shown. Note that the expected values for all 4 plasma samples were ∼4,000 copies/mL.