Table 1.

The top ranking genes mediating resistance to fludarabine identified in the piggyBac screen

Gene (HGNC symbol)DescriptionTTAA sitesExpected insertionsObserved insertionsCell poolP valueP value FDR
SNX5Sorting nexin 52350.02817. 2800
MGME1Mitochondrial genome maintenance exonuclease 11720.02817. 2800
EIF2AK3Eukaryotic translation initiation factor 2-alpha kinase 36340.04617. 286.86E−141.57E−11
DCKDeoxycytidine kinase3480.034174.24E−103.88E−08
SLC1A3Solute carrier family 1 (glial high affinity glutamate transporter), member 36090.044171.43E−091.19E−07
RBM34RNA binding motif protein 341840.023176.68E−095.11E−07
TRIM31Tripartite motif containing 31760.012284.00E−082.62E−06
NUDT8Nudix (nucleoside diphosphate linked moiety X)-type motif 850.001281.00E−076.11E−06
BMP2KBMP2 inducible kinase14150.144284.31E−071.96E−05
MOB1AMOB kinase activator 1A2010.0142284.71E−071.96E−05
  • NOTE: The ranking was based on frequency of observed transposon integrations in relation to number of total potential piggyBac insertion sites (4 nt recognition site, TTAA) within the genes and 5 kb upstream of the genes. Only protein encoding genes expressed in HG3 cells, according to the RNA-sequencing data (normalized FPKM value greater than 0.1), were considered as plausible candidates. The P values were calculated using Poisson statistics, with false discovery rate (FDR) correction for multiple tests and a corrected P value < 0.05 as cutoff for selection of significant genes.

  • Abbreviation: HGNC, HUGO Gene Nomenclature Committee.