Table 1

Antibodies and TMA scoring criteria

MarkerSourceDilutionPretreatment
1ERDAKO1:100Steam 20 min EDTA
2PRDAKO1:100Steam 20 min EDTA
3Her 2DAKO1:500Steam 20 min, TRS
4Her 3Neomarker1:400Steam 30 min, citrate buffer (pH 6.0)
5Her 4Neomarker1:160Steam 30 min, citrate buffer (pH 6.0)
6p53DAKO1:400Pressure cooker in MV, 2 min
7p63Neomarker1:1000Steam 30 min, citrate buffer (pH 6.0)
8RelaxinImmune Diagnostics1:100Steam 30 min, citrate buffer (pH 6.0)
9E2F1Neomarker1:200Microwave 15 min, citrate buffer (pH 6.0)
10Ki67Novocastra1:100Pressure cooker in MV, 2 min
11Cyclin ENeomarker1:320Microwave, 30 min, citrate buffer (pH 6.0)
12PTEN * 1:300Microwave, 30 min, citrate buffer (pH 6.0)
13CA IX[31]1:50Steam 30 min, citrate buffer (pH 6.0)
14HSP27Novocastra1:800Steam 30 min, citrate buffer (pH 6.0)
15IGFBP2Santa Cruz1:200Steam 30 min, citrate buffer (pH 6.0)
16IGFBP5Santa Cruz1:300Steam 30 min, citrate buffer (pH 6.0)
17Cox2Cayman Chemical1:100Steam 20 min, citrate buffer (pH 6.0)
18CK5/6Chemicon1:100Pressure cooker in MV, 2 min
19YB1 1:2000Steam 30 min, citrate buffer (pH 6.0)
20E-cadherinIntermedico-Zymed1:150Decloak 5 min TRS
21TIP1[32]§1:80Citrate buffer (ph 6.0), 5 min
22CD3Novocastra1:600Decloak 5 min, EDTA
23CD10Novocastra1:50Decloak 5 min TRS
24CD20DAKO1:5000.05% pronase
25CD43Quartett1:10Decloak 5 min TRS
26CD68DAKO1:800Decloak 5 min TRS
27CD117DAKO1:50None
28Osteo-protegerin 1:2500None
29NSEDAKO1:100None
30Chromogranin ABiogenex1:1000None
31SynaptophysinDAKO1:200None