Table 1A

Continued.

Staining patternScore 0Score 1Score 2Score 3
Nuclear10%10 to <30%30 to <70%≥70%
Nuclear<10%10 to <30%30 to <70%≥70%
MembranousNegativeHerceptest score 1N/aHerceptest scores 2 and 3
CytoplasmicNegativeWeakIntermediateStrong
CytoplasmicNegativeN/aN/aPositive
Nuclear<10%10 to <50%N/a≥50%
Nuclear≤2 cells>3 cells to <10%N/a≥10%
Extracellular matrixNegativeWeakIntermediateStrong
Nuclear<1%1 to <10%≥10 to 30%>30%
Nuclear< 10%10 to 30%N/a>30%
Nuclear<10%10 to 50%N/a>50%
CytoplasmicNegativeWeakIntermediateStrong
Membranous≤1 cell>1 cell to <30%N/a≥30%
Membranous≤1 cell>1 cell to <30%N/a≥30%
CytoplasmicNegativeWeakIntermediateStrong
CytoplasmicNegativeWeakIntermediateStrong
CytoplasmicNegativeWeakIntermediateStrong
CytoplasmicNegativeWeakIntermediateStrong
CytoplasmicNegativeWeakIntermediateStrong
CytoplasmicNegativeWeakIntermediateStrong
CytoplasmicNegativeWeakIntermediateStrong
Stromal T cell<10 cellN/aN/a≥10 cell
Stromal fibroblastsNegativeWeakIntermediateStrong
Stromal B cell<10 cellN/aN/a≥10 cell
Stromal T cell<10 cellN/aN/a≥10 cell
Stromal macrophages<10 cellN/aN/a≥10 cell
Stromal mast cellNegativeN/aN/a≥1 cell
NuclearNegative10 to 50%N/a>50%
CytoplasmicNegativeWeakIntermediateStrong
CytoplasmicNegativeWeak/focalIntermediateStrong
CytoplasmicNegativeWeak/focalIntermediateStrong
  • Note. Manufacturer’s locations are, as follows: DAKO (Glostrup, Denmark); Neumarker (Fremont, CA); Immune Diagnostics (Buffalo, NY); Novocastra (Newcastle, UK); Santa Cruz (Santa Cruz, CA); Cayman Chemical (Ann Arbor, MI); Chemicon (Temecula, CA); Intermedico-Zymed (San Francisco, CA), Quartett (Berlin, Germany), and Biogenex (San Ramon, CA).

  • Abbreviations: TRS, Tris; N/a, not applicable; MV, microwave; NSE, neuron-specific enolase.

  • * The Anti-PTEN is a polyclonal antibody provided as a gift from Dr. Heung-Chin Cheng, University of Melbourne, Melbourne, Victoria, Australia.

  • A gift from Dr. Stephen Chia, British Columbia Cancer Agency, Vancouver, BC, Canada.

  • A gift from Dr. Colin Nelson, Prostate Centre of Vancouver General Hospital, Vancouver, BC, Canada.

  • § A gift from Dr. Calvin Roskelley, University of British Columbia, Vancouver, BC, Canada.

  • Gift from the University of Sheffield, Sheffield, United Kingdom.