Table 1

The differentially expressed proteins between acute leukemia and NWBCs identified by MALDI-TOF-MS

SpotProtein nameNCBInr ID no.MrpIPeptides (MALDI/MS)Sequence coverage (%)ScoreProtein expression
TheorObservTheorObservMatchTotal
N-1*Proliferating cell nuclear antigen (PCNA)gi 450564128,75029,4654.574.4910364568+
N-2PTK9L protein tyrosine kinase 9-like (A6-related protein);gi 600584639,52335,6746.376.895112263+
N-3Pyridoxine 5′-phosphate oxidasegi 892249829,96922,3256.626.76173452161+
N-4*Uracil DNA glycosylasegi 3505335,47038,6668.226.676142464+
N-5*Stathmin; leukemia-associated phosphoprotein p18, (Op18)gi 503185117,29219,2315.765.5514576099+
N-6Guanine nucleotide binding protein (G protein)gi 517444735,05535,1037.607.87122755137+
N-7Rho GDP dissociation inhibitor (GDI) αgi 475776823,19323,3675.025.0011616288+
N-8TNF inhibitory protein (TIP)gi 1377519810,43110,5314.824.7814576099+
N-9Voltage-dependent anion channel 2gi 1527757730,39330,0346.816.729394288+
N-10Mitochondrial short-chain enoyl-coenzyme A hydratase 1 precursorgi 1270757031,35128,5648.346.01194062151+
N-11NM23-H1gi 2946818419,64121,0455.835.5211458097+
N-12Apoptosis inhibitor homologgi 213478056,61729,9766.225.878192175+
N-13Similar to proteasome (prosome, macropain) subunit, α type, 2gi 1280409525,13824,1757.927.687214583+
N-14Peroxisomal enoyl-CoA hydratase 1gi 450344735,97138,4566.616.878223171+
N-15*High mobility group box 1 (HMGB 1)gi 450442524,87822,9875.626.0013354773+
N-16*Glutathione S-transferase–like; glutathione S-transferase omega (GST)gi 475863825,01925,1326.006.218394782
N-17*Chain A, manganese superoxide dismutase (MnSOD)gi 278081822,17623,2186.867.0111616288
N-18Similar to homo sapiens mRNA for KIAA0120 gene with GenBank accession no. D21261.1gi 995602624,43821,4658.415.23205374176
N-19Myosin regulatory light chain 3 (MLC3)gi 545374019,78120,9744.674.4010276297
N-20Myosin regulatory light chain (MLC2)gi 1580901619,76720,1964.674.616234572
N-21ras suppressor protein 1gi 691263831,52130,5678.577.9815504588
N-22Chain A, dimeric form of the haemopexin domain of Mmp9gi 2320014622,40720,8698.768.568192274
N-23Chain B, crystal structure of A Rac-Rho gdi complexgi 995520620,46415,6876.165.64114155104
N-24Lactoferringi 38685530,56323,6958.148.019253576
N-25S100 calcium-binding protein A12gi 503205910,56911,5365.835.425123982
N-26Chain A, carboxylic ester hydrolase, P 1 21 1 space groupgi 2320032713,62212,5425.105.13465666
N-27Chain A, crystal structure of the Mrp14 complexed with Chapsgi 2015022913,10214,2315.715.459387697
N-28CFAg (AA 1–94)gi 2988810,93113,8959.195.927527178
N-29S100 calcium-binding protein A8 (Mrp8)gi 2161454410,82810,7326.517.067295989
  • NOTE. The calculation of experimental isoelectric point (pI) and molecular weight (Mr) was based on migration of the protein spot on two-dimensional gels. Differentially expressed proteins between acute leukemia and NWBCs were designed with the letter N. “+” indicates the up-regulated proteins in AL. “−” indicates the down-regulated proteins in AL. A score more than 63 is significant (P < 0.05). The protein spots were numbered in the order in which they were discovered in our work.

  • Abbreviations: ID, identification; Theor, theoretical; Observ, observed; TNF, tumor necrosis factor.

  • * The proteins that had been reported to be involved in leukemogenesis.