Table 3

Relationship between cell homozygosity, retinoic acid receptor-β2 (RAR-β2) expression, methylation status, and p53 mutations in head and neck squamous cell carcinoma cell lines

No.Cell linePrimary siteNo. of 3p24 lociaRAR-β2 statusbMethylation statuscp53 mutationd
1UMSCC10ALarynx2U+
2UMSCC10BLarynx2+U+
3UMSCC17ALarynx1M
4UMSCC17BLarynx1MM
5MDA886LnLarynxNDe+M
6UMSCC11BHypopharynx1+U+
7UMSCC22AHypopharynx1M
8UMSCC22BHypopharynx1MM
9UMSCC35Oropharynx1MM+
10UMSCC14BFloor of mouth1+U+
11UMSCC19Base of tongue1U+
12UMSCC30Pyriform sinus1+U+
13UMSCC38Tonsil1MM
14183ATonsilND+U+
151186EpiglottisNDM+
161483Retromolar trigoneND+U
17SqCC/Y1Buccal mucosaNDM+
18TR146Buccal mucosaNDMM
  • a One copy of DNA at the 3p24 locus (29 , 45 46 47) .

  • b RAR-β2 expression level as detected using both Northern blotting and reverse transcription-PCR analysis and before treatment.

  • c U, unmethylated; M, partially methylated with a mix of methylated and unmethylated alleles; MM, high-density, >80% methylated alleles.

  • d Absence (−) or presence (+) of p53 mutation (57) .

  • e ND, not determined.