Table 2

Details of the antibodies used

MMPaAntibody namePretreatmentDilutionControl
MMP1: 36632 monoclonalInterstitial collagenaseNone1:100Duodenum
MMP2: 36033 monoclonalGelatinase ANone1:100Smooth muscle
MMP3: RP58 monoclonalStromelysin 1Trypsin (20′)1:50Kidney
MMP7: RP21 polyclonalMatrilysinNone1:100Skeletal muscle
MMP8: SE603 polyclonalNeutrophil collagenaseNone1:200 to 1:400Skeletal muscle
MMP9: 4H3 polyclonalGelatinase BTrypsin (20′)1:100Appendix
MMP11 monoclonalStromelysin 3None1:100Breast cancer
MMP12: RP54 monoclonalMetalloellastaseNone1:400Kidney
MMP14: RP63 monoclonalMT-MMP1None1:200Smooth muscle
TIMP 1 monoclonal147-6D11Microwave1:200Pancreas
TIMP 2 monoclonal67-4H11Trypsin (20′)1:200Colon
TIMP 3 monoclonal136-13H4Microwave1:1000Colon
  • a MMP, matrix metalloproteinases; TIMP, tissue inhibitor of the metalloproteinases.