Table 3

Immunohistochemical staining of pancreatic tissue

MMP or TIMPaPancreatic ductal adenocarcinomaNormal pancreasCancer vs. normalPrognostic significance
Antibody for:Staining intensityCarcinomaStromaAdjacent pancreatitisaEpitheliaStroma
MMP-1016 (36%)Nil38 (85%)7 (70%)NilNSNS
N = 45 PDAC,111 (24%)2 (4%)
N = 10 normal215 (33%)5 (11%)3 (30%)
33 (7%)
MMP-2025 (33%)bNil35 (47%)3 (30%)NilNSNS
N = 75 PDAC,121 (28%)9 (12%)4 (40%)
N = 10 normal213 (17%)17 (23%)3 (30%)
316 (21%)14 (19%)
MMP-305 (11%)18 (40%)13 (29%)stroma vs. stroma, P < 0.0001NS
N = 45 PDAC,116 (36%)22 (49%)1 (2%)10 (100%)
N = 10 normal212 (27%)5 (11%)31 (69%)10 (100%)
312 (27%)0
MMP-704 (9%)bNil19 (42%)8 (80%)NilP < 0.0001P < 0.005
N = 45 PDAC,113 (29%)6 (13%)2 (20%)
N = 10 normal219 (42%)16 (35%)
39 (20%)4 (11%)
MMP-8012 (27%)dNil2 (8%)4 (40%)NilP = 0.04NS
N = 45 PDAC,118 (40%)9 (36%)6 (60%)
N = 10 normal213 (29%)13 (52%)
32 (4%)1 (4%)
MMP-9026 (58%)NilNil10 (100%)NilP = 0.0009NS
N = 45 PDAC,113 (29%)
N = 10 normal23 (7%)
33 (7%)
MMP-11023 (31%)53 (71%)35 (47%)8 (80%)NilP = 0.004P = 0.02
N = 75 PDAC,120 (27%)18 (24%)9 (12%)2 (20%)(RT-PCR, P = 0.0005)
N = 10 normal218 (24%)3 (4%)17 (23%)
314 (19%)1 (1%)14 (19%)
MMP-12025 (56%)Nil20 (44%)NilNSNS
N = 45 PDAC,119 (42%)20 (44%)8 (80%)
N = 10 normal25 (11%)2 (20%)
31 (2%)
MMP-14031 (41%)b,c,dNil37 (49%)6 (60%)NilNSNS
N = 75 PDAC,126 (35%)22 (29%)4 (40%)
N = 10 normal212 (16%)15 (20%)
36 (8%)1 (1%)
TIMP-1042 (93%)NilNil10 (100%)NilNS (RT-PCR, P = 0.03)NS
N = 45 PDAC,1
N = 10 normal23 (7%)
3
TIMP-2025 (56%)Nil17 (49%)2 (20%)NilNSNS
N = 45 PDAC,117 (38%)14 (40%)8 (80%)
N = 10 normal21 (2%)4 (11%)
32 (4%)
TIMP-307 (9%)21 (28%)8 (11%)2 (20%)NilP < 0.0001, stroma vs. stroma, P < 0.0003NS (P = 0.06)
N = 75 PDAC,113 (17%)9 (12%)13 (17%)8 (80%)
N = 10 normal226 (35%)17 (23%)26 (35%)
329 (39%)28 (37%)28 (37%)
  • a MMP, matrix metalloproteinases; TIMP, tissue inhibitor of the metalloproteinases; NS, not significant; RT, reverse transcription; PDAC, pancreatic durtal adenocarcinoma.

  • b MMP-2 correlated with MMP-14 (P < 0.0001).

  • c MMP-7 correlated with MMP-14 (P = 0.0005).

  • d MMP-8 correlated with MMP-14 (P < 0.0001). NS = not significant.

  • e Not all tumor sections had adjacent pancreatitis, percentage expressed as fraction of scored adjacent pancreatitis.