Table 1.

Primers and probes for genotyping single-nucleotide polymorphisms

Target gene (Genbank accession no.) and amino acid exchangeSequence of primers or probes*
XRCC1 (L34079), Arg194TrpF: 5′-TACTGACCTTGCGGGACCTTA-3′
R: 5′-TACCCTCAGACCCACGAGTCTA-3′
S: 5′-TGTCTTGTTGATCCAGCTGAAGAAG-FITC-3′
A: 5′-LCRed640-AGCCCCCGGCCTCAGAGAGTTGGp-3′
XRCC1 (L34079), Arg280HisF: 5′-GCTGGGGCCTGGATTG-3′
R: 5′-GCACCACTACCACACCCTGAA-3′
S: 5′-TGCCAGCTCCAACTCATACCCC-FITC-3′
A: 5′-LCRed640-CCACAGCCCCAGTCCCTGCCCGp-3′
XRCC1 (L34079), Arg399GlnF: 5′-CCCCAAGTACAGCCAGGTC-3′
R: 5′-TGTCCCGCTCCTCTCAGTAG-3′
S: 5′-CCCTCCCAGAGGTAAGGCC-FITC-3′
A: 5′-LCRed640-CACACGCCAACCCTGCTCCTTATp-3′
APE1 (M92444), Asp148GluF: 5′-CTTGATTGCTTTCCCTTTTCTTA-3′
R: 5′-CGCTGCCGGTACTCCA-3′
S: 5′-LCRed640-TGCTCCTCCTCGCCTATAGAAATGAp-3′
A: 5′-CACAATCACCCGGCCTTCCTGATC-FITC-3′
XPD (L47234), Asp312AsnF: 5′-GACAGACGAGCAGCGCC-3′
R: 5′-AAGCCCAGGAAATGCTCG-3′
S: 5′-CCCGTGCTGCCCAACGAA-FITC-3′
A: 5′-LCRed640-TGCTGCAGGGTGAGCCCCGACCp-3′
XPD (L47234), Lys751GlnF: 5′-GCTCAGCCTGGAGCAGCTA-3′
R: 5′-GTGAGAAATGTCACCTGACTTCAT-3′
S: 5′-LCRed640-CAATCTGCTCTATCCTCTGCAGCGTCp-3′
A: 5′-CCCACCCGCCCCACTCAGAGCTGCT-FITC-3′
  • Abbreviations: F, forward primer; R, reverse primer; S, sensor; A, anchor.

  • * Mutation site is shaded.